Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, TN 38163, USA; Neuroscience Institute, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, TN 38163, USA; Neuroscience Institute, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
STAR Protoc. 2022 Dec 16;3(4):101722. doi: 10.1016/j.xpro.2022.101722. Epub 2022 Sep 24.
Stable and accurate capturing of detailed social behaviors is essential for studying rodent sociability. Here, we introduce a round social arena (RSA) system that enables close-up monitoring of detailed social behaviors in mice. We describe the steps to build RSA apparatus and set up the wiring for video synchronization. We then detail how to conduct RSA experiment with simultaneous Ca imaging or optogenetics. This protocol also includes a custom MATLAB script for aligning the behavioral dataset to Ca trace data. For complete details on the use and execution of this protocol, please refer to Kim et al. (2022).
稳定而准确地捕捉详细的社交行为对于研究啮齿动物的社交能力至关重要。在这里，我们介绍了一种圆形社交竞技场（RSA）系统，可实现对小鼠详细社交行为的近距离监测。我们描述了构建 RSA 设备和进行视频同步布线的步骤。然后，我们详细介绍了如何进行同时进行钙成像或光遗传学的 RSA 实验。该方案还包括一个用于将行为数据集与钙轨迹数据对齐的自定义 MATLAB 脚本。有关此方案的使用和执行的完整详细信息，请参阅 Kim 等人。（2022 年）。
