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纤维光度测定与啮齿类动物模型中社会行为整合的方案。

Protocol for the integration of fiber photometry and social behavior in rodent models.

机构信息

Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada.

Department of Psychology, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada; Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB T2N 1N4, Canada.

出版信息

STAR Protoc. 2023 Dec 15;4(4):102689. doi: 10.1016/j.xpro.2023.102689. Epub 2023 Nov 18.

DOI:10.1016/j.xpro.2023.102689
PMID:37979176
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10694594/
Abstract

Fiber photometry offers insight into cell-type-specific activity underlying social interactions. We provide a protocol for the integration of fiber photometry recordings into the analysis of social behavior in rodent models. This includes considerations during surgery, notes on synchronizing fiber photometry with behavioral recordings, advice on using multi-animal behavioral tracking software, and scripts for the analysis of fiber photometry recordings. For complete details on the use and execution of this protocol, please refer to Dawson et al. (2023)..

摘要

光纤光度法可以深入了解社交互动背后的细胞类型特异性活动。我们提供了一个将光纤光度法记录整合到啮齿动物模型的社会行为分析中的方案。这包括手术期间的注意事项、将光纤光度法与行为记录同步的说明、使用多动物行为跟踪软件的建议以及光纤光度法记录分析的脚本。有关该方案使用和执行的完整详细信息,请参阅 Dawson 等人(2023 年)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b664/10694594/dc15b9b3fd61/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b664/10694594/6fb56a261dca/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b664/10694594/f9eb09e93b87/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b664/10694594/bc38938a459a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b664/10694594/dc15b9b3fd61/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b664/10694594/6fb56a261dca/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b664/10694594/f9eb09e93b87/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b664/10694594/bc38938a459a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b664/10694594/dc15b9b3fd61/gr3.jpg

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1
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STAR Protoc. 2023 Dec 15;4(4):102689. doi: 10.1016/j.xpro.2023.102689. Epub 2023 Nov 18.
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