Srisuthtayanont Warunee, Pruksakorn Dumnoensun, Kongtawelert Prachya, Pothacharoen Peraphan
Department of Biochemistry, Thailand Excellence Center for Tissue Engineering and Stem Cells, Faculty of Medicine, Chiang Mai University, 110 Intavaroros Road, Sripoom, Muang, Chiang Mai, 50200, Thailand.
Department of Orthopedics, Orthopedic Laboratory and Research Netting Center (OLARN Center), Faculty of Medicine, Chiang Mai University, Intavaroros Road, Sripoom, Muang, Chiang Mai, 50200, Thailand.
BMC Complement Altern Med. 2017 May 31;17(1):286. doi: 10.1186/s12906-017-1805-1.
Numerous studies have reported on the health benefits of sesamin, a major lignin found in sesame (S. indicum) seeds. Recently, sesamin was shown to have the ability to promote chondroitin sulfate proteoglycan synthesis in normal human chondrocytes. This study assesses the anti-inflammatory effect of sesamin on proteoglycans production in 3D chondrocyte cultures.
To evaluate the effects of sesamin on IL-1β-treated human articular chondrocytes (HAC) pellets, the pellets were pre-treated with IL-1β then cultured in the presence of various concentrations of sesamin for 21 days. During that period, the expression of IL-1β, glycosaminoglycans (GAGs) content and Chondroitin sulfate proteoglycans (CSPGs) synthesis genes (ACAN, XT-1, XT-2, CHSY1 and ChPF) was measured. The GAGs accumulation in the extracellular matrix was determined on day 21 by histological analysis.
There was clear evidence that sesamin upregulated expression of all the CSPGs synthesis genes, in contrast to the down-regulation of IL-1β expression both in genes and in protein levels. The level of release and matrix accumulation of GAGs in IL-1β pre-treated HAC pellets in the presence of sesamin was recovered. These results correlate with the histological examination which showed that sesamin enhanced matrix CSPGs accumulation.
Sesamin enhances CSPGs synthesis, suppresses IL-1β expression and ameliorates IL-1β induced inflammation in human chondrocytes. Sesamin could have therapeutic benefits for treating inflammation in osteoarthritis.
大量研究报道了芝麻素（一种存在于芝麻种子中的主要木质素）对健康的益处。最近，研究表明芝麻素能够促进正常人软骨细胞中硫酸软骨素蛋白聚糖的合成。本研究评估芝麻素对三维软骨细胞培养物中蛋白聚糖产生的抗炎作用。
为了评估芝麻素对白细胞介素-1β（IL-1β）处理的人关节软骨细胞（HAC）微球的影响，先将微球用IL-1β预处理，然后在不同浓度的芝麻素存在下培养21天。在此期间，检测IL-1β的表达、糖胺聚糖（GAGs）含量以及硫酸软骨素蛋白聚糖（CSPGs）合成基因（ACAN、XT-1、XT-2、CHSY1和ChPF）的表达。在第21天通过组织学分析确定细胞外基质中GAGs的积累情况。
有明确证据表明，芝麻素上调了所有CSPGs合成基因的表达，与之相反的是，IL-1β在基因和蛋白质水平上均表达下调。在芝麻素存在的情况下，IL-1β预处理的HAC微球中GAGs的释放水平和基质积累得以恢复。这些结果与组织学检查结果相关，组织学检查显示芝麻素增强了基质CSPGs的积累。
芝麻素增强CSPGs合成，抑制IL-1β表达，并改善IL-1β诱导的人软骨细胞炎症。芝麻素可能对治疗骨关节炎炎症具有治疗益处。
