Pan Wenfei, Gao He, Ying Xiaoling, Xu Caiju, Ye Xiang, Shao Yelin, Hua Mengdi, Shao Jie, Zhang Xinxue, Fu Shaowei, Yang Min
Department of Nutrition and Food Hygiene School of Public Health, and Center of Clinical Big Data and Analytics of The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Zhejiang Provincial Centre for Disease Control and Prevention, Hangzhou, China.
Front Nutr. 2022 Sep 7;9:997006. doi: 10.3389/fnut.2022.997006. eCollection 2022.
This study aimed to investigate anemia treatment and other potential effects of two food-derived bioactive oligopeptide iron complexes on pregnant rats with iron deficiency anemia (IDA) and their offspring. Rats with IDA were established with a low iron diet and then mated. There were one control group and seven randomly assigned groups of pregnant rats with IDA: Control group [Control, 40 ppm ferrous sulfate (FeSO)]; IDA model group (ID, 4 ppm FeSO), three high-iron groups (H-FeSO, 400 ppm FeSO; MCOP-Fe, 400 ppm marine fish oligopeptide iron complex; WCOP-Fe, 400 ppm whey protein oligopeptide iron complex) and three low-iron groups (L-FeSO, 40 ppm FeSO; MOP-Fe, 40 ppm marine fish oligopeptide iron complex; WOP-Fe, 40 ppm whey protein oligopeptide iron complex). Rats in each group were fed the corresponding special diet during pregnancy until the day of delivery. After different doses of iron supplement, serum hemoglobin, iron, and ferritin levels in rats with IDA were significantly increased to normal levels ( < 0.05). Serum iron levels were significantly lower in two food-derived bioactive oligopeptide low-iron complex groups than in the low FeSO group (<0.05). Liver malondialdehyde levels were significantly increased in the three high-iron groups compared with the other five groups ( < 0.05), and hemosiderin deposition was observed in liver tissue, indicating that the iron dose was overloaded and aggravated the peroxidative damage in pregnant rats. Liver inflammation was reduced in the three low-iron groups. Tumor necrosis factor α secretion was significantly decreased in all groups with supplemented oligopeptide ( < 0.05), with the concentration of tumor necrosis factor α declining to normal levels in the two whey protein oligopeptide iron complex groups. In the marine fish oligopeptide iron complex groups, body length, tail length, and weight of offspring were significantly increased ( < 0.05) and reached normal levels. Therefore, food-derived bioactive oligopeptide (derived from marine fish skin and milk) iron complexes may be an effective type of iron supplement for pregnancy to improve anemia, as well as reduce the side effects of iron overload, and improve the growth and nutritional status of offspring.
本研究旨在探讨两种食物来源的生物活性寡肽铁络合物对缺铁性贫血（IDA）孕鼠及其后代的贫血治疗效果和其他潜在影响。通过低铁饮食建立IDA大鼠模型，然后使其交配。将患有IDA的孕鼠分为1个对照组和7个随机分组：对照组[Control，40 ppm硫酸亚铁（FeSO）]；IDA模型组（ID，4 ppm FeSO），3个高铁组（H-FeSO，400 ppm FeSO；MCOP-Fe，400 ppm海鱼寡肽铁络合物；WCOP-Fe，400 ppm乳清蛋白寡肽铁络合物）和3个低铁组（L-FeSO，40 ppm FeSO；MOP-Fe，40 ppm海鱼寡肽铁络合物；WOP-Fe，40 ppm乳清蛋白寡肽铁络合物）。每组大鼠在孕期喂食相应的特殊饮食直至分娩。补充不同剂量的铁后，IDA大鼠的血清血红蛋白、铁和铁蛋白水平显著升高至正常水平（<0.05）。两种食物来源的生物活性寡肽低铁络合物组的血清铁水平显著低于低剂量FeSO组（<0.05）。与其他五组相比，三个高铁组的肝脏丙二醛水平显著升高（<0.05），并且在肝组织中观察到含铁血黄素沉积，表明铁剂量过载并加重了孕鼠的过氧化损伤。三个低铁组的肝脏炎症减轻。所有补充寡肽的组中肿瘤坏死因子α分泌显著降低（<0.05），在两个乳清蛋白寡肽铁络合物组中肿瘤坏死因子α浓度降至正常水平。在海鱼寡肽铁络合物组中，后代的体长、尾长和体重显著增加（<0.05）并达到正常水平。因此，食物来源的生物活性寡肽（源自海鱼皮和牛奶）铁络合物可能是孕期改善贫血的一种有效铁补充剂，同时还能减少铁过载的副作用，并改善后代的生长和营养状况。
