Granovskiy Dmitriy L, Ryabchevskaya Ekaterina M, Evtushenko Ekaterina A, Kondakova Olga A, Arkhipenko Marina V, Kravchenko Tatiana B, Bakhteeva Irina V, Timofeev Vitalii S, Nikitin Nikolai A, Karpova Olga V
Department of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology (FBIS SRCAMB), Obolensk, Russia.
Front Microbiol. 2022 Sep 9;13:1003969. doi: 10.3389/fmicb.2022.1003969. eCollection 2022.
Anthrax is a disease caused by . The most promising approach to the development of anthrax vaccine is use of the anthrax protective antigen (PA). At the same time, recombinant PA is a very unstable protein. Previously, the authors have designed a stable modified recombinant anthrax protective antigen with inactivated proteolytic sites and substituted deamidation sites (rPA83m). As a second approach to recombinant PA stabilisation, plant virus spherical particles (SPs) were used as a stabiliser. The combination of these two approaches was shown to be the most effective. Here, the authors report the results of a detailed study of the stability, immunogenicity and protectiveness of rPA83m + SPs compositions. These compositions were shown to be stable, provided high anti-rPA83m antibody titres in guinea pigs and were able to protect them from a fully virulent 81/1 strain. Given these facts, the formulation of rPA83m + SPs compositions is considered to be a prospective anthrax vaccine candidate.
炭疽病是由……引起的一种疾病。开发炭疽疫苗最有前景的方法是使用炭疽保护性抗原（PA）。与此同时，重组PA是一种非常不稳定的蛋白质。此前，作者设计了一种具有失活蛋白水解位点和取代脱酰胺位点的稳定修饰重组炭疽保护性抗原（rPA83m）。作为重组PA稳定化的第二种方法，植物病毒球形颗粒（SPs）被用作稳定剂。这两种方法的结合被证明是最有效的。在此，作者报告了对rPA83m + SPs组合物的稳定性、免疫原性和保护性进行详细研究的结果。这些组合物被证明是稳定的，在豚鼠中产生了高抗rPA83m抗体滴度，并能够保护它们免受完全有毒力的81/1菌株的侵害。鉴于这些事实，rPA83m + SPs组合物的配方被认为是一种有前景的炭疽疫苗候选物。
