基于血清的蛋白质组学对结直肠癌的诊断价值评估。
Evaluation of the diagnostic value of serum-based proteomics for colorectal cancer.
作者信息
Wang Hui-Juan, Xie Yi-Bin, Zhang Peng-Jun, Jiang Tao
机构信息
Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Beijing Institute of Respiratory Medicine, Capital Medical University, Beijing 100020, China.
Department of Pancreatic and Gastric Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
出版信息
World J Gastrointest Oncol. 2022 Aug 15;14(8):1562-1573. doi: 10.4251/wjgo.v14.i8.1562.
BACKGROUND
Colorectal cancer (CRC) is a highly malignant cancer with a high incidence and mortality in China. It is urgent to find a diagnostic marker with higher sensitivity and specificity than the traditional approaches for CRC diagnosis.
AIM
To provide new ideas for the diagnosis of CRC based on serum proteomics.
METHODS
Specimens from 83 healthy people, 62 colon polyp (CRP) patients, and 101 CRC patients were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The diagnostic value of the profiles of differentially expressed proteins was then analyzed.
RESULTS
Compared with the healthy control group, CRC patients had elevated expression of 5 proteins and reduced expression of 14 proteins. The area under the curve (AUC) for a differentially expressed protein with a mass-to-charge ratio of 2022.34 was the largest; the AUC was 0.843, which was higher than the AUC of 0.717 observed with carcinoembryonic antigen (CEA), and the sensitivity and specificity of this identified marker were 75.3% and 79.5%, respectively. After cross-validation, the accuracy of diagnosis using levels of this differentially expressed protein was 82.37%. Compared with the CRP group, the expression of 3 proteins in the serum of CRC patients was elevated and 11 proteins were expressed at reduced levels. Proteins possessing mass-to-charge ratio values of 2899.38 and 877.3 were selected to establish a classification tree model. The results showed that the accuracy of CRC diagnosis was 89.5%, the accuracy of CRP diagnosis was 81.6%, and the overall accuracy of this approach was 86.3%. The overall sensitivity and specificity of diagnosis using the proteomics approach were 81.8% and 66.75%, respectively. The sensitivities and specificities of diagnoses based on CEA and carbohydrate antigen 19-9 expression were 55.6% and 91.3% and 65.4% and 65.2%, respectively.
CONCLUSION
We demonstrated that serum proteomics may be helpful for the detection of CRC, and it may assist clinical practice for CRC diagnosis.
背景
结直肠癌（CRC）是一种高度恶性的癌症，在中国发病率和死亡率都很高。迫切需要找到一种比传统方法具有更高敏感性和特异性的诊断标志物用于CRC诊断。
目的
基于血清蛋白质组学为CRC诊断提供新思路。
方法
采用基质辅助激光解吸/电离飞行时间质谱法分析83名健康人、62名结肠息肉（CRP）患者和101名CRC患者的样本。然后分析差异表达蛋白质谱的诊断价值。
结果
与健康对照组相比，CRC患者有5种蛋白质表达升高，14种蛋白质表达降低。质荷比为2022.34的差异表达蛋白质的曲线下面积（AUC）最大；AUC为0.843，高于癌胚抗原（CEA）观察到的AUC 0.717，该鉴定标志物的敏感性和特异性分别为75.3%和79.5%。交叉验证后，使用该差异表达蛋白质水平进行诊断的准确率为82.37%。与CRP组相比，CRC患者血清中3种蛋白质表达升高，11种蛋白质表达降低。选择质荷比为2899.38和877.3的蛋白质建立分类树模型。结果显示，CRC诊断准确率为89.5%，CRP诊断准确率为81.6%，该方法的总体准确率为86.3%。蛋白质组学方法诊断的总体敏感性和特异性分别为81.8%和66.75%。基于CEA和糖类抗原19-9表达诊断的敏感性和特异性分别为55.6%和91.3%以及65.4%和65.2%。
结论
我们证明血清蛋白质组学可能有助于CRC的检测，并可能辅助CRC诊断的临床实践。
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