Kim Yangmin X, Son Su Young, Lee Seulbi, Lee Yejin, Sung Jwakyung, Lee Choong Hwan
National Institute of Agricultural Sciences, Rural Development Administration, Wanju, South Korea.
Department of Bioscience and Biotechnology, Konkuk University, Seoul, South Korea.
Front Plant Sci. 2022 Sep 8;13:983725. doi: 10.3389/fpls.2022.983725. eCollection 2022.
Effect of water supply to metabolites in tomato fruit was compared in two soils with different nutrient conditions, i.e., either limited or excess. Two types of soil nutrient condition, type A: nutrient-limited and type B: nutrient-excess, were prepared as follows; type A is a low nutrient-containing soil without a replenishment of starved nitrogen and phosphorous, type B is a high nutrient-containing soil exceeding the recommended fertilization. Soil water was adjusted either at -30 kPa (sufficient) or -80 kPa (limited). For harvested tomato fruits, we examined primary and secondary metabolites using non-targeted mass spectrometry based metabolomics. The fruit production and leaf SPAD were greatly dependent on soil nutrient levels, by contrast, the level of lycopene remained unchanged by different levels of water and nutrient supply. The perturbation of metabolites by water supply was clear in the nutrient-excess soil. In particular, limited water supply strongly decreased primary metabolites including sugars and amino acids. We demonstrated that water stress differently shifted primary metabolites of tomato fruits in two soils with different nutrient conditions non-targeted mass spectrometry-based metabolomics. In conclusion, we suggest that the limited water supply in soils with surplus nutrient is not a recommendable way for tomato 'cv. Super Dotaerang' production if fruit nutritional quality such as sugars and amino acids is in the consideration, although there was no disadvantage in fruit yield.
在两种养分条件不同（即有限或过量）的土壤中，比较了水分供应对番茄果实代谢物的影响。制备了两种类型的土壤养分条件，A 型：养分有限，B 型：养分过量，具体如下；A 型是一种低养分含量的土壤，不补充饥饿的氮和磷，B 型是一种高养分含量的土壤，超过了推荐施肥量。土壤水分调节为-30 kPa（充足）或-80 kPa（有限）。对于收获的番茄果实，我们使用基于非靶向质谱的代谢组学方法检测了初级和次级代谢物。果实产量和叶片 SPAD 值在很大程度上取决于土壤养分水平，相比之下，不同水平的水分和养分供应对番茄红素水平没有影响。在养分过量的土壤中，水分供应对代谢物的扰动很明显。特别是，有限的水分供应强烈降低了包括糖类和氨基酸在内的初级代谢物。我们通过基于非靶向质谱的代谢组学方法证明，水分胁迫在两种养分条件不同的土壤中对番茄果实初级代谢物的影响不同。总之，我们建议，如果考虑果实的营养品质，如糖类和氨基酸，那么对于番茄‘cv. Super Dotaerang’的生产来说，在养分过剩的土壤中供应有限的水分不是一种可取的方法，尽管这对果实产量没有不利影响。
