Lengfelder E, Fuchs C, Younes M, Weser U
Biochim Biophys Acta. 1979 Apr 12;567(2):492-502. doi: 10.1016/0005-2744(79)90135-9.
The superoxide dismutative action of Cu-penicillamine was examined by pulse radiolysis. The second order rate constand of the reaction wpith superoxide was 0.4 +/- o.2.10(9) M-1.s-1, comparable to the action of Fe and Mn-superoxide dismutases. No marked pH-dependence was seen. Neither ethylene diamine tetraacetic acid nor cyanide affected the catalytic action of Cu-penicillamine. The cyanide resistant reactivity as well as further X-ray photoelectron spectrometric measurements supported the suggestion of a Cu(I) stabilized sulphur radical being the active species involved in the catalysis of superoxide dismutation.
通过脉冲辐解研究了铜-青霉胺的超氧化物歧化作用。与超氧化物反应的二级速率常数为0.4±0.2×10⁹ M⁻¹·s⁻¹，与铁和锰超氧化物歧化酶的作用相当。未观察到明显的pH依赖性。乙二胺四乙酸和氰化物均不影响铜-青霉胺的催化作用。抗氰反应性以及进一步的X射线光电子能谱测量结果支持了以下观点：即铜（I）稳定的硫自由基是参与超氧化物歧化催化作用的活性物种。
