Götz F, Elstner E F, Sedewitz B, Lengfelder E
Arch Microbiol. 1980 Apr;125(3):215-20. doi: 10.1007/BF00446879.
Cell-free extracts of Lactobacillus plantarum contain non-proteinaceous compounds which mimic superoxide dismutase activity. Using the test system in which O-2 is generated by xanthine oxidase, superoxide dismutase activity is found in cell-free extracts, where proteins are removed by precipitation. This activity is strongly decreased after dialysis of cell-free extracts. Superoxide dismutase activity was also investigated by means of pulse radiolysis. Cell-free extracts of Escherichia coli were also investigated as a comparison, which were known to contain superoxide dismutase. With cell-free extracts of both L. plantarum and E. coli the decay of O-2 was markedly increased. However, the type of reaction of the O-2 decay was of first order in the presence of E. coli extracts due to superoxide dismutase(s), and of second order in the presence of L. plantarum extracts, indicating that O-2 elimination is not an enzymic reaction. Mn2+ phosphate(s) might be responsible for the observed elimination of O-2. The production of O-2 is not detectable during NADH-, lactate- or pyruvate oxidase reactions in L. plantarum extracts.
植物乳杆菌的无细胞提取物含有模拟超氧化物歧化酶活性的非蛋白质化合物。在黄嘌呤氧化酶产生O₂的测试系统中，发现无细胞提取物中存在超氧化物歧化酶活性，其中的蛋白质通过沉淀被去除。无细胞提取物透析后，这种活性显著降低。还通过脉冲辐解研究了超氧化物歧化酶活性。作为对照，还研究了已知含有超氧化物歧化酶的大肠杆菌的无细胞提取物。植物乳杆菌和大肠杆菌的无细胞提取物都使O₂的衰减明显增加。然而，在大肠杆菌提取物存在下，由于超氧化物歧化酶的作用，O₂衰减的反应类型为一级反应，而在植物乳杆菌提取物存在下为二级反应，这表明O₂的消除不是酶促反应。锰的磷酸盐可能是观察到的O₂消除的原因。在植物乳杆菌提取物的NADH、乳酸或丙酮酸氧化酶反应过程中，未检测到O₂的产生。
