Correlation between and in clinical strains of at planktonic and biofilm states.

This study aimed to explore the influences of and on itraconazole (ITR) resistance of at different states. A total of 10 ITR-resistant strains and 10 ITR-sensitive strains were used for sequencing and sequencing. sequencing showed no missense mutation, and three synonymous mutations. gene sequencing identified two missense mutations M140I (8) and K191Q (4), and 14 synonymous mutations G201A (1), A246C (5), C282T (6), G288A (6), C321T (7), A399C (16), C432T (16), C465T (11), G552A (16), G669T (1), G672A (1), G681T (2), T783C (1), and T819A (2). The biofilm formation capacity of resistant strains, including the ∆/∆ strain, was stronger. Afterward, real-time quantitative PCR was used to analyze the expression of and . Compared with the sensitive strains, and expressions were both significantly upregulated in resistant strains at planktonic and biofilm states ( < 0.05). Compared with the strains at planktonic state, was significantly upregulated, while was significantly downregulated at biofilm states ( < 0.05). Additionally, expression in the knocked down strain of was significantly upregulated, and expression was evidently downregulated in the ∆/∆ strain at biofilm states compared with that at planktonic states ( < 0.05). Loss of can increase level and may influence the biofilm formation of , thus increasing ITR resistance of.