State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, College of Food and Pharmaceutical Sciences, Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315832, Zhejiang Province, PR China.
Zhejiang Academy of Agricultural Sciences, Institute Of Agro-product Safety And Nutrition, Hangzhou, Zhejiang Province, PR China.
Food Chem. 2023 Feb 15;402:134231. doi: 10.1016/j.foodchem.2022.134231. Epub 2022 Sep 14.
The correlation of antioxidant activity and prebiotic effect about oat phenolic compounds was invested, while there exists limited studies. Free and bound phenolic compounds were separated from ethyl acetate, n-butanol and aqueous fractions. Fluorescence in situ hybridization was used to investigate gut microbiota of in vitro fermentation samples. The results showed that ethyl acetate fraction possesses higher total phenolics contents than that of aqueous fraction (p < 0.01). The bound-n-butanol and free-ethyl acetate fraction exhibited the higher antioxidant capacity (p < 0.01). The phenolic compounds with more powerful antioxidant capacity could promote the proliferation of gut microbiota (Lactobacillus/Enterococcus spp. and Bifidobacterium spp.) (p < 0.05) and inhibit the growth of gut microbiota (Bacteroides spp. and Clostridium/histolyticum group). There is a positive correlation of antioxidant activity and prebiotic effect about oat phenolic compounds. This study provides a basis for the correlation between antioxidant stress and gut microbiota regulation in vivo.
研究了燕麦酚类化合物的抗氧化活性和益生元作用的相关性，然而相关研究十分有限。从乙酸乙酯、正丁醇和水相部分分离出游离和结合酚类化合物。使用荧光原位杂交技术研究体外发酵样品中的肠道微生物群。结果表明，乙酸乙酯部分的总酚含量高于水相部分（p<0.01）。结合正丁醇和游离乙酸乙酯部分表现出更高的抗氧化能力（p<0.01）。具有更强抗氧化能力的酚类化合物可以促进肠道微生物群（乳杆菌/肠球菌属和双歧杆菌属）的增殖（p<0.05），并抑制肠道微生物群（拟杆菌属和梭菌/组织溶菌素组）的生长。燕麦酚类化合物的抗氧化活性和益生元作用之间存在正相关关系。本研究为体内抗氧化应激与肠道微生物群调节之间的相关性提供了依据。
