Koga M, Hori H, Tanaka I, Akiyama T, Inoue N
J UOEH. 1987 Jun 1;9(2):167-70. doi: 10.7888/juoeh.9.167.
Urinary metabolites of rats exposed to ethylene oxide(EO) were analyzed by gas chromatography-mass spectrometry(GC/MS). Male Wister rats(220-240 g) were exposed to EO for 6 hours. The urine was collected during 18 hours after the exposure and extracted with ethyl acetate. For the water-soluble metabolites, ethanol was added to the urine centrifuged and the supernatant fraction was evaporated to dryness. The residue of the extract was methylated with diazomethane and trimethylsilylated with bis(trimethylsilyl)trifluoroacetamide. Ethylene glycol, 2-hydroxymercapturic acid, 2-methylthioethanol and 2-mercaptoethanol were identified as the metabolites of EO. These results suggest that the inhaled EO was hydrolyzed to ethylene glycol, and conjugated with glutathion to form the mercapturic acid and methylthio metabolite.
采用气相色谱 - 质谱联用（GC/MS）法分析暴露于环氧乙烷（EO）的大鼠的尿液代谢产物。雄性Wister大鼠（220 - 240克）暴露于EO 6小时。暴露后18小时内收集尿液，并用乙酸乙酯萃取。对于水溶性代谢产物，向离心后的尿液中加入乙醇，将上清液部分蒸发至干。提取物的残留物用重氮甲烷进行甲基化，并用双（三甲基硅基）三氟乙酰胺进行三甲基硅基化。乙二醇、2 - 羟基巯基尿酸、2 - 甲硫基乙醇和2 - 巯基乙醇被鉴定为EO的代谢产物。这些结果表明，吸入的EO被水解为乙二醇，并与谷胱甘肽结合形成巯基尿酸和甲硫基代谢产物。
