通过高压液相色谱法和气相色谱-质谱联用技术测定血清和尿液中的乙醇酸来诊断犬乙二醇（防冻液）中毒。

Diagnosis of ethylene glycol (antifreeze) intoxication in dogs by determination of glycolic acid in serum and urine with high pressure liquid chromatography and gas chromatography-mass spectrometry.

作者信息

Hewlett T P, Ray A C, Reagor J C

出版信息

J Assoc Off Anal Chem. 1983 Mar;66(2):276-83.

Abstract

A relatively fast and sensitive high pressure liquid chromatographic (HPLC) and gas chromatographic-mass spectrometric (GC/MS) method was developed for determination of glycolic acid, one of the major metabolites of ethylene glycol, in extracts of canine urine and serum. The procedure involves extraction of glycolic acid with methyl ethyl ketone and derivatization with O-p-nitrobenzyl-N,N'-diisopropylisourea (PNBDI) in ethyl acetate solution. Recovery was greater than 94% from spiked samples. Ethylene glycol and commercial antifreeze were administered to experimental dogs at different dosage levels to reproduce the naturally occurring toxicosis associated with the consumption of commercial antifreeze. Glycolic acid was determined in either the urine or serum or both from these dogs by HPLC and GC/MS. 1,3-Butanediol, a competitive inhibitor of ethylene glycol biotransformation, was administered to one dog concurrently with antifreeze. In that experiment, it was effective in decreasing glycolic acid formation and prevented acute metabolic acidosis, kidney damage, and death.

摘要

建立了一种相对快速且灵敏的高压液相色谱（HPLC）和气相色谱 - 质谱联用（GC/MS）方法，用于测定犬尿液和血清提取物中乙醇酸（乙二醇的主要代谢产物之一）。该方法包括用甲乙酮萃取乙醇酸，并在乙酸乙酯溶液中用O - 对硝基苄基 - N，N'-二异丙基异脲（PNBDI）进行衍生化。加标样品的回收率大于94%。以不同剂量水平给实验犬施用乙二醇和市售防冻液，以重现与食用市售防冻液相关的自然发生的中毒情况。通过HPLC和GC/MS测定这些犬尿液或血清或两者中的乙醇酸。给一只犬同时施用1,3 - 丁二醇（乙二醇生物转化的竞争性抑制剂）和防冻液。在该实验中，它有效地减少了乙醇酸的形成，并预防了急性代谢性酸中毒、肾损伤和死亡。