JC 多瘤病毒在甲状腺乳头状癌中的踪迹：伊朗的综合研究。
Traces of JC polyomavirus in papillary thyroid cancer: a comprehensive study in Iran.
机构信息
Department of Biotechnology, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Molecular and Cellular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
出版信息
Virol J. 2022 Sep 26;19(1):153. doi: 10.1186/s12985-022-01881-4.
BACKGROUND
JC polyomavirus (JCPyV) is known to induce solid tumors such as astrocytomas, glioblastomas, and neuroblastomas in experimental animals, and recent studies have shown that the virus may be correlated with carcinogenesis. This study aimed to evaluate the impact of JCPyV on the progression of papillary thyroid cancer (PTC).
METHODS
A total of 1057 samples, including 645 paraffin-embedded PTC biopsy samples (PEBS) and 412 fresh biopsy samples (FBS), and 1057 adjacent non-cancerous samples were evaluated for the presence of JCPyV DNA and RNA.
RESULTS
We observed that 10.8% (114/1057) samples, including 17.5% (72/412) FBS and 6.5% (42/645) PEBS were positive for the JCPyV DNA. Among the JCPyV-positive samples, the mean JCPyV copy number was lower in patients with PEBS (0.3 × 10 ± 0.1 × 10 copies/cell) compared to FBS (1.8 × 10 ± 0.4 × 10 copies/cell) and non-PTC normal samples (0.2 × 10 ± 0.01 × 10 copies/cell), with a statistically significant difference (P < 0.001). The LT-Ag RNA expression was lower in PEBS than in FBS, while no VP1 gene transcript expression was found.
CONCLUSIONS
Although our results confirmed the presence of JCPyV in some Iranian patients with PTC, more research is needed to verify these results.
背景
JC 多瘤病毒（JCPyV）已知会在实验动物中诱导星形细胞瘤、胶质母细胞瘤和神经母细胞瘤等实体肿瘤，最近的研究表明该病毒可能与致癌作用有关。本研究旨在评估 JCPyV 对甲状腺乳头状癌（PTC）进展的影响。
方法
共评估了 1057 个样本，包括 645 个石蜡包埋 PTC 活检样本（PEBS）和 412 个新鲜活检样本（FBS）以及 1057 个相邻非癌性样本，以评估 JCPyV DNA 和 RNA 的存在情况。
结果
我们发现，10.8%（114/1057）的样本，包括 17.5%（72/412）的 FBS 和 6.5%（42/645）的 PEBS 为 JCPyV DNA 阳性。在 JCPyV 阳性样本中，PEBS 中 JCPyV 拷贝数平均值低于 FBS（0.3×10±0.1×10 拷贝/细胞）和非 PTC 正常样本（0.2×10±0.01×10 拷贝/细胞），差异具有统计学意义（P<0.001）。LT-Ag RNA 表达在 PEBS 中低于 FBS，而未检测到 VP1 基因转录表达。
结论
尽管我们的结果证实了一些伊朗 PTC 患者存在 JCPyV，但需要进一步研究来验证这些结果。
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