Department of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Urology, Golestan Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Braz J Microbiol. 2020 Mar;51(1):37-43. doi: 10.1007/s42770-019-00117-y. Epub 2019 Jul 30.
JC polyomavirus (JCPyV) is the causative agent for progressive multifocal leukoencephalopathy (PML) in immunocompromised patients. More than 40% of healthy population excretes JCPyV particles in their urine. As JCPyV is ubiquitous in human, the definition of genotype distribution can help trace population migration. In this study, to define the frequency of JCPyV in southwest of Iran, urine samples of 161 volunteers including 80 healthy individuals and 81 HIV-infected patients were collected. PCR assays and sequence analysis were performed using JCPyV-specific primers designed against VP1 coding region. JCPyV DNA was detected in 65 out of 81 urine samples (80.2%) of HIV-infected, and in 43 out of 80 urine samples (53.8%) of healthy individuals (P = 0.001). The shedding of JCPyV among HIV-infected patients revealed an age-related pattern while such relationship was not observed in healthy individuals group. The most common genotype found in this region was genotype 3A (80.8%), followed by genotype 2D (11.5%), 4 (3.8%), and 7 (3.8%). The frequency of JCPyV in the urine of HIV-infected patients was found significantly higher than in the healthy individuals (P = 0.001).
JC 多瘤病毒 (JCPyV) 是免疫功能低下患者进行性多灶性白质脑病 (PML) 的病原体。超过 40%的健康人群会在尿液中排出 JCPyV 颗粒。由于 JCPyV 在人类中普遍存在，基因型分布的定义可以帮助追踪人群迁移。在这项研究中，为了确定伊朗西南部 JCPyV 的流行频率，收集了 161 名志愿者的尿液样本，包括 80 名健康个体和 81 名 HIV 感染患者。使用针对 VP1 编码区设计的 JCPyV 特异性引物进行 PCR 检测和序列分析。在 81 名 HIV 感染患者的 65 份尿液样本（80.2%）和 80 名健康个体的 43 份尿液样本（53.8%）中检测到 JCPyV DNA（P=0.001）。HIV 感染患者 JCPyV 的排出呈现出与年龄相关的模式，而在健康个体组中未观察到这种关系。在该地区发现的最常见基因型是基因型 3A（80.8%），其次是基因型 2D（11.5%）、4（3.8%）和 7（3.8%）。HIV 感染患者尿液中 JCPyV 的频率明显高于健康个体（P=0.001）。
