Zhai En-Ai, Mi Wen-Juan, Cui Yang, Hong Wei-Feng, Wang Ya-Shun, Guo Xing-Yu, Zou Hui-Qin, Yan Yong-Hong
School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
Jiangsu Simcere Pharmaceutical Co., Ltd. Nanjing 210042, China.
Zhongguo Zhong Yao Za Zhi. 2022 Sep;47(17):4600-4608. doi: 10.19540/j.cnki.cjcmm.20220515.101.
This study aims to explore the consistency between macroscopic identification and DNA barcoding identification of Amomi Fructus. With the DNA barcoding identification results, we evaluated the reliability of identifying Amomi Fructus quality by combining macroscopic traits with main volatile chemical components. Thirteen batches of Amomi Fructus samples were collected for identification. Firstly, the morphological and sensory characteristics of each sample were observed and recorded according to the standard in Chinese Pharmacopoeia(2020 edition). The 100-fruit weight, longitudinal diameter, transverse diameter, and longitudinal diameter-to-transverse diameter ratio were measured, which correspond to large, solid, and full kernel representing good quality in the sensory evaluation. The odor value detected by electronic nose and major volatile components(borneol, camphor, limonene, and borneol acetate) correspond to the sensory evaluation of strong odor representing good quality. Secondly, DNA barcoding was employed to identify the 13 batches of samples. Finally, clustering analysis was performed for the main volatile components and macroscopic traits, and the identification results were compared with those of DNA barcoding. Except two batches of samples(No.6 and No.10), the macroscopic identification showed the results consistent with those of DNA barcoding, with an identification rate of 84.62%. The clustering results of the content of four volatile chemical components and macroscopic traits were also consistent with the DNA barcoding identification results. DNA barcoding can verify the results of macroscopic identification and provide a scientific basis for the inheritance and development of macroscopic identification. Moreover, the combination of macroscopic traits and chemical components demonstrates higher accuracy in the quality evaluation of Chinese medicinal materials.
本研究旨在探讨砂仁宏观鉴定与DNA条形码鉴定之间的一致性。依据DNA条形码鉴定结果，我们通过结合宏观特征与主要挥发性化学成分来评估砂仁质量鉴定的可靠性。收集了13批砂仁样品进行鉴定。首先，按照《中国药典》（2020年版）标准观察并记录每个样品的形态和感官特征。测量百果重、纵径、横径以及纵横径比，在感官评价中，这些指标对应着果实大、坚实饱满且果仁充实代表质量良好。通过电子鼻检测的气味值以及主要挥发性成分（冰片、樟脑、柠檬烯和乙酸龙脑酯）对应着气味浓郁代表质量良好的感官评价。其次，采用DNA条形码对这13批样品进行鉴定。最后，对主要挥发性成分和宏观特征进行聚类分析，并将鉴定结果与DNA条形码的鉴定结果进行比较。除两批样品（6号和10号）外，宏观鉴定结果与DNA条形码鉴定结果一致，鉴定率为84.62%。四种挥发性化学成分含量与宏观特征的聚类结果也与DNA条形码鉴定结果一致。DNA条形码可以验证宏观鉴定结果，为宏观鉴定的传承与发展提供科学依据。此外，宏观特征与化学成分相结合在中药材质量评价中显示出更高的准确性。
