Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Int J Mol Sci. 2024 Aug 19;25(16):9005. doi: 10.3390/ijms25169005.
The genus includes over 111 species, 6 of which are widely utilized as medicinal plants and have already undergone taxonomic revision. Due to their morphological similarities, the presence of counterfeit and substandard products remains a challenge. Accurate plant identification is, therefore, essential to address these issues. This study utilized 11 newly sequenced samples and extensive NCBI data to perform molecular identification of the six medicinal "Doukou" species. The plastomes of these species exhibited a typical quadripartite structure with a conserved gene content. However, independent variation shifts of the SC/IR boundaries existed between and within species. The comprehensive set of genetic sequences, including ITS, ITS1, ITS2, complete plastomes, K, L, A-H, and 1, showed varying discrimination of the six "Doukou" species based on both distance and phylogenetic tree methods. Among these, the ITS, ITS1, and complete plastome sequences demonstrated the highest identification success rate (3/6), followed by 1 (2/6), and then ITS2, K, and A-H (1/6). In contrast, L failed to identify any species. This research established a basis for a reliable molecular identification method for medicinal "Doukou" plants to protect wild plant resources, promote the sustainable use of medicinal plants, and restrict the exploitation of these resources.
该属包括超过 111 种，其中 6 种被广泛用作药用植物，并已经经历了分类修订。由于它们形态相似，假冒伪劣产品的存在仍然是一个挑战。因此，准确的植物鉴定对于解决这些问题至关重要。本研究利用 11 个新测序的样本和广泛的 NCBI 数据，对六种药用“独活”物种进行了分子鉴定。这些物种的质体基因组显示出典型的四分体结构，具有保守的基因含量。然而，在种间和种内，SC/IR 边界的独立变异存在。包括 ITS、ITS1、ITS2、完整质体基因组、K、L、A-H 和 1 在内的综合遗传序列，根据距离和系统发育树方法，对六种“独活”物种进行了不同的区分。其中，ITS、ITS1 和完整质体基因组序列的鉴定成功率最高（3/6），其次是 1（2/6），然后是 ITS2、K 和 A-H（1/6）。相比之下，L 未能鉴定出任何物种。这项研究为药用“独活”植物的可靠分子鉴定方法奠定了基础，以保护野生植物资源，促进药用植物的可持续利用，并限制这些资源的开发。
