从一个携带与双相情感障碍和复发性抑郁障碍相关的 1p36-35 单体型的家族中生成四条 iPSC 系：三代患者和一个健康的同胞。
Generation of four iPSC lines from a family harboring a 1p36-35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling.
机构信息
Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Neuropsychiatry, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.
Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan.
出版信息
Stem Cell Res. 2022 Oct;64:102915. doi: 10.1016/j.scr.2022.102915. Epub 2022 Sep 14.
Induced pluripotent stem cells (iPSCs) obtained from genetically characterized patients benefit the biological study of bipolar disorder (BD). Here, we present iPSC lines from three-generation patients with BD and recurrent depressive disorder (RDD) and a healthy control sibling in a family. All patients shared the specified haplotype in the 1p36-35, previously reported as the susceptibility locus of mood disorders. iPSCs were generated with the reprogramming factors OTC3/4, l-MYC, LIN28, SOX2, KLF4, and p53 shRNA through non-integrated episomal vectors. All iPSC lines strongly expressed pluripotency markers and proved the ability to differentiate into three germ lineages in vitro.
诱导多能干细胞（iPSC）来源于具有遗传特征的患者，这有利于双相障碍（BD）的生物学研究。在这里，我们介绍了来自一个三代 BD 患者和复发性抑郁障碍（RDD）患者以及一个健康对照兄弟姐妹的 iPSC 系。所有患者在 1p36-35 上共享特定的单倍型，这是先前报道的情绪障碍易感位点。通过非整合的附加体载体，使用重编程因子 OTC3/4、l-MYC、LIN28、SOX2、KLF4 和 p53 shRNA 生成 iPSC。所有 iPSC 系均强烈表达多能性标记物，并证明了在体外分化为三个胚层谱系的能力。
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