从两位患有精神分裂症和双相情感障碍的表兄弟及其未受影响的表兄弟中生成和鉴定人类诱导多能干细胞系。
Generation and characterization of human-derived iPSC lines from two cousins with schizophrenia and bipolar disorder and their unaffected cousin.
机构信息
The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Department of Neurobiology, Affiliated Mental Health Center & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine, Hangzhou 310013, Zhejiang, China; NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Science and Brain-machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
出版信息
Stem Cell Res. 2022 Aug;63:102832. doi: 10.1016/j.scr.2022.102832. Epub 2022 Jun 7.
Schizophrenia (SCZ) and bipolar disorder (BD) are debilitating neurodevelopmental disorders with high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by three females. An adolescent female was clinically diagnosed as first-episode SCZ. One of her cousins was clinically diagnosed as BD and another one was unaffected control. Induced pluripotent stem cells (iPSCs) were established with reprograming factors Oct4, Sox2, Nanog, Lin28, c-myc, Klf4, and SV40LT. All lines presented normal karyotype and highly expressed pluripotency markers in vitro. All iPSCs were capable to differentiate into derivatives of three germ layers in vivo.
精神分裂症 (SCZ) 和双相情感障碍 (BD) 是具有高度遗传性的使人衰弱的神经发育障碍。在这项研究中，采集了三名女性的外周血单核细胞 (PBMCs)。一名青春期女性被临床诊断为首发精神分裂症。她的一位表亲被临床诊断为双相情感障碍，另一位表亲则未受影响。使用重编程因子 Oct4、Sox2、Nanog、Lin28、c-myc、Klf4 和 SV40LT 建立诱导多能干细胞 (iPSC)。所有细胞系均表现出正常的核型，并在体外高度表达多能性标志物。所有 iPSC 均能在体内分化为三个胚层的衍生物。
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