纳米胶囊化技术提高了微藻三角褐指藻提取物中叶黄醇的生物利用度。

Nanoencapsulation enhances the bioavailability of fucoxanthin in microalga Phaeodactylum tricornutum extract.

机构信息

Natural Product Informatics Center, KIST Gangneung Institute of Natural Products, Gangneung 25451, Republic of Korea.

Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, Republic of Korea.

出版信息

Food Chem. 2023 Mar 1;403:134348. doi: 10.1016/j.foodchem.2022.134348. Epub 2022 Sep 21.

DOI:10.1016/j.foodchem.2022.134348

PMID:36166925

Abstract

The marine carotenoid fucoxanthin (FX) has various health benefits but suffers from poor bioavailability. We hypothesize that the bioavailability of FX in microalga Phaeodactylum tricornutum extract (PE) could be improved through nanoencapsulation. Here, we developed two types of nanoparticles: one consisting of alginate and casein (A-C-PE, 246 nm diameter, 79.6% encapsulation efficiency) and the other A-C-PE coated with chitosan (CS-A-C-PE, 258 nm, 78.1%). Both types of nanoparticles incorporating PE showed controlled FX release during simulated gastrointestinal digestion, as well as 1.8-fold improvement of membrane permeability in Caco-2/TC7 cells compared to non-encapsulated PE. Pharmacokinetic behavior of two FX metabolites (fucoxanthinol and amarouciaxanthin A) in mouse plasma was monitored after oral administration. The results showed that 31.8-332.1% more FX metabolites from the nanoparticles were absorbed into plasma than those from PE. In conclusion, encapsulation of PE in both types of nanoparticles significantly promoted the bioavailability of FX.

摘要

海洋类胡萝卜素岩藻黄质 (FX) 具有多种健康益处，但生物利用度较差。我们假设通过纳米封装可以提高微藻三角褐指藻提取物 (PE) 中 FX 的生物利用度。在这里，我们开发了两种类型的纳米颗粒：一种由海藻酸钠和酪蛋白组成 (A-C-PE，直径 246nm，包封效率 79.6%），另一种由 A-C-PE 包覆壳聚糖 (CS-A-C-PE，直径 258nm，包封效率 78.1%）。这两种类型的纳米颗粒均含有 PE，在模拟胃肠道消化过程中表现出对 FX 的控制释放，并且与未封装的 PE 相比，在 Caco-2/TC7 细胞中的膜通透性提高了 1.8 倍。在口服给药后监测了两种 FX 代谢物（岩藻黄质醇和amarouciaxanthin A）在小鼠血浆中的药代动力学行为。结果表明，与 PE 相比，来自纳米颗粒的 FX 代谢物有 31.8-332.1%被更多地吸收到血浆中。总之，两种类型的纳米颗粒对 PE 的封装显著提高了 FX 的生物利用度。