Laboratory of Biology (BIO@SNS), Scuola Normale Superiore, 56126, Pisa, Italy.
Department of Veterinary Sciences, University of Pisa, 56124, Pisa, Italy.
Cold Spring Harb Protoc. 2022 Dec 1;2022(12):624-629. doi: 10.1101/pdb.prot107746.
Organotypic culture is a well-established method for culturing ex vivo tissue samples. The advantages of culturing tissue slices for prolonged time periods ex vivo are numerous and consist primarily of the maintenance of the overall in vivo architecture of the isolated sample, the lack of the ematoencephalic barrier, and the ease of pharmacological treatments and interventions that can be conducted under controlled conditions as in in vitro systems such as cell cultures. Given the extremely short life span of and the emergence of aging signs only after a few months of life, it is of particular interest to establish this protocol for as a potential method to study brain aging ex vivo.
器官型培养是一种成熟的体外培养组织样本的方法。将组织切片在体外长时间培养具有许多优势，主要包括保持分离样本的整体体内结构、不存在血脑屏障，以及便于在体外系统（如细胞培养）中进行的药物治疗和干预措施。鉴于 的寿命极短，并且只有在几个月的生命后才会出现衰老迹象，因此建立这种 体外脑衰老研究的方法具有特别的意义。
