Department of Psychoneuropharmacology, Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
Department of Genetic Collections of Neural Disorders, Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
Int J Mol Sci. 2023 Feb 6;24(4):3185. doi: 10.3390/ijms24043185.
The annual turquoise killifish () is a laboratory model organism for neuroscience of aging. In the present study, we investigated for the first time the levels of serotonin and its main metabolite, 5-hydroxyindoleacetic acid, as well as the activities of the key enzymes of its synthesis, tryptophan hydroxylases, and degradation, monoamine oxidase, in the brains of 2-, 4- and 7-month-old male and female . The marked effect of age on the body mass and the level of serotonin, as well as the activities of tryptophan hydroxylases and monoamine oxidase in the brain of killifish were revealed. The level of serotonin decreased in the brain of 7-month-old males and females compared with 2-month-old ones. A significant decrease in the tryptophan hydroxylase activity and an increase in the monoamine oxidase activity in the brain of 7-month-old females compared to 2-month-old females was shown. These findings agree with the age-related alterations in expression of the genes encoding tryptophan hydroxylases and monoamine oxidase. is a suitable model with which to study the fundamental problems of age-related changes of the serotonin system in the brain.
年度绿松石食蚊鱼（）是衰老神经科学的实验室模式生物。在本研究中，我们首次研究了 2、4 和 7 月龄雄性和雌性食蚊鱼脑中 5-羟色胺及其主要代谢物 5-羟吲哚乙酸的水平，以及其合成的关键酶色氨酸羟化酶和降解酶单胺氧化酶的活性。年龄对鱼体质量和 5-羟色胺水平以及脑中色氨酸羟化酶和单胺氧化酶活性的显著影响被揭示出来。与 2 月龄相比，7 月龄雄性和雌性食蚊鱼脑中的 5-羟色胺水平降低。与 2 月龄雌性相比，7 月龄雌性食蚊鱼脑中的色氨酸羟化酶活性显著降低，单胺氧化酶活性升高。这些发现与编码色氨酸羟化酶和单胺氧化酶的基因表达的年龄相关变化一致。食蚊鱼是研究脑中 5-羟色胺系统与年龄相关变化的基本问题的合适模型。
