Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Department of Traditional Medicine, Faculty of Persian Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Int J Neurosci. 2024 Jun;134(6):570-583. doi: 10.1080/00207454.2022.2130293. Epub 2022 Oct 10.
Traditional Persian medicine has introduced effective remedies in opioid dependence care. One of the most widely used remedies is an herbal formulation containing L. and L. (HF). This study investigated the effects of HF to attenuate the withdrawal signs and rewarding effects in morphine-dependent rats. Forty-nine male Wistar rats were randomly divided into seven groups. The control and vehicle groups received normal saline and sodium carboxymethyl cellulose, respectively. The morphine group received morphine for one week. The single and daily dose of HF groups received morphine similar to the morphine group, and HF (1.4 and 2.8 g/kg) once a day in the daily dose group and only on the last day of the experiment in the single dose of HF group. Finally, the withdrawal signs as well biochemical tests were evaluated. The behavioral parameters were assessed by conditioned place preference (CPP), elevated plus-maze and Y-maze tests. The antioxidant activity of HF was evaluated by measurement of serum contents of malondialdehyde, stable nitric oxide metabolites and total antioxidant capacity (TAC). Moreover, the protein expression of c-fos was assessed by western blotting. Daily treatment with HF significantly reduced the score of CPP behavioral test, all of the withdrawal signs, TAC and the c-fos protein level. The results indicated that HF might be a promising complementary treatment in reducing morphine-induced physical and psychological dependence probably through modulation of c-fos protein expression.
传统波斯医学在阿片类药物依赖治疗中引入了有效疗法。其中一种应用广泛的疗法是一种草药配方，其中包含 L. 和 L. (HF)。本研究旨在探究 HF 对减轻吗啡依赖大鼠戒断症状和奖赏效应的影响。49 只雄性 Wistar 大鼠随机分为 7 组。对照组和载体组分别给予生理盐水和羧甲基纤维素钠。吗啡组接受吗啡治疗一周。HF 单剂量组和每日剂量组接受与吗啡组相似的吗啡，每日剂量组每日给予 HF（1.4 和 2.8 g/kg），单剂量组仅在实验的最后一天给予 HF。最后评估了戒断症状和生化指标。通过条件性位置偏好（CPP）、高架十字迷宫和 Y 迷宫试验评估行为参数。通过测量血清丙二醛、稳定的一氧化氮代谢物和总抗氧化能力（TAC）来评估 HF 的抗氧化活性。此外，通过 Western blot 评估 c-fos 蛋白的表达。HF 的每日治疗显著降低了 CPP 行为测试的评分、所有戒断症状、TAC 和 c-fos 蛋白水平。结果表明，HF 可能是一种有前途的辅助治疗方法，可通过调节 c-fos 蛋白表达来减轻吗啡引起的身体和心理依赖。
