Hadadi Maryam, Farazi Mohammad Mojtaba, Mehrabani Mehrnaz, Tashakori-Miyanroudi Mahsa, Behroozi Zahra
Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
Sci Rep. 2025 Apr 15;15(1):12910. doi: 10.1038/s41598-025-93726-7.
About 70% of spinal cord injury (SCI) patients experience neuropathic pain (NP), posing an important medical challenge. Painkillers are used to manage pain today and often have undesirable side effects. Curcumin's antioxidant properties may help alleviate NP following SCI (NP-SCI). We decided to study curcumin's effects on NP-SCI for the first time. Male Wistar rats were divided into five groups (n = 8): Control (no injury/no treatment), Sham (laminectomy), SCI (spinal cord compression at T11-T12 using a clip), Curcumin100 and Curcumin200 (Curcumin at 100 and 200 mg/kg administered 30 min after SCI for 10days). Motor function, allodynia, and hyperalgesia were assessed using the BBB scale, acetone, and tail-flick until six weeks after SCI. H&E staining for assaying cavity, western blot for measuring GAD65 and GABA-A receptors, and biochemical kits for assaying SOD, catalase, total antioxidant capacity, and MDA were used. PRISM software analyzed data. Results showed significant improvements in motion, allodynia, hyperalgesia, cavity, urinary retention (P < 0.0001), and weight in curcumin treatments. There was also a reduction in MDA, with increasing GABA-A receptors, GAD65, and antioxidants in them. Findings suggest curcumin may provide good analgesic effects through its antioxidants, and extensive studies are needed to confirm it as a treatment for NP-SCI in the clinic. Keyboards: spinal cord injury, curcumin, antioxidant, pain, GABA, GAD65 enzyme.
约70%的脊髓损伤（SCI）患者会经历神经性疼痛（NP），这构成了一项重大医学挑战。如今，止痛药被用于控制疼痛，但往往伴有不良副作用。姜黄素的抗氧化特性可能有助于缓解脊髓损伤后的神经性疼痛（NP-SCI）。我们首次决定研究姜黄素对NP-SCI的影响。将雄性Wistar大鼠分为五组（n = 8）：对照组（无损伤/未治疗）、假手术组（椎板切除术）、SCI组（使用夹子在T11-T12水平压迫脊髓）、姜黄素100组和姜黄素200组（脊髓损伤后30分钟给予100和200 mg/kg姜黄素，持续10天）。使用BBB评分、丙酮和甩尾试验评估运动功能、痛觉过敏和痛觉超敏，直至脊髓损伤后六周。采用苏木精-伊红（H&E）染色检测空洞，蛋白质免疫印迹法检测GAD65和GABA-A受体，生化试剂盒检测超氧化物歧化酶（SOD）、过氧化氢酶、总抗氧化能力和丙二醛（MDA）。使用PRISM软件分析数据。结果显示，姜黄素治疗组在运动、痛觉过敏、痛觉超敏、空洞、尿潴留（P < 0.0001）和体重方面有显著改善。MDA也有所降低，同时GABA-A受体、GAD65和抗氧化剂含量增加。研究结果表明，姜黄素可能通过其抗氧化剂发挥良好的镇痛作用，需要进行广泛研究以证实其在临床上作为NP-SCI治疗方法的有效性。关键词：脊髓损伤；姜黄素；抗氧化剂；疼痛；GABA；GAD65酶
