Hunter R H, Fléchon B, Fléchon J E
Tissue Cell. 1987;19(3):423-36. doi: 10.1016/0040-8166(87)90037-1.
Using sexually mature animals, the distribution of spermatozoa has been examined at the utero-tubal junction and in the distal and proximal portions of the oviduct isthmus. Mating occurred during early oestrus and, with one exception, specimens were prepared shortly before or after ovulation. Distinct reservoirs of spermatozoa were identified in furrows between the terminal folds of the isthmus, and particularly within the troughs and transverse ridges of this region. The density of spermatozoa diminished steeply from the utero-tubal junction towards the isthmus, especially in the pre-ovulatory specimens. The membranes of most spermatozoa in the isthmus were intact up to the time of ovulation, suggesting that the acrosome reaction is a peri- or post-ovulatory event. Whilst the flagella of spermatozoa in the reservoirs were usually straight or only slightly curved, those on the surface of the epithelial folds were undulating (S-shaped). Specific microenvironments may therefore exist in the distal portion of the isthmus to regulate sperm motility; droplets of secretion were a notable feature in this region. In specimens prepared 24 hr after ovulation, spermatozoa were almost absent from the utero-tubal junction and isthmus. However, denuded eggs were observed in the proximal portion of the isthmus in this animal, and they had spermatozoa associated with the zona pellucida. Arguments are presented for a peri-ovulatory endocrine regulation of sperm redistribution and capacitation.
利用性成熟动物，研究了子宫输卵管连接处以及输卵管峡部远端和近端的精子分布情况。交配发生在发情初期，除一个例外，标本在排卵前不久或排卵后不久制备。在峡部末端皱襞之间的沟中，特别是在该区域的凹槽和横向嵴内，发现了明显的精子储存库。从子宫输卵管连接处向峡部，精子密度急剧下降，尤其是在排卵前的标本中。直到排卵时，峡部大多数精子的膜仍保持完整，这表明顶体反应是排卵周围或排卵后的事件。储存库中精子的鞭毛通常是直的或仅略有弯曲，而上皮皱襞表面的鞭毛则呈波浪状（S形）。因此，峡部远端可能存在特定的微环境来调节精子活力；分泌液滴是该区域的一个显著特征。在排卵后24小时制备的标本中，子宫输卵管连接处和峡部几乎没有精子。然而，在该动物峡部近端观察到了裸卵，并且它们的透明带上有精子附着。本文提出了关于排卵周围内分泌对精子重新分布和获能的调节的观点。
