转录组分析表明，手术治疗可能会影响 III/IV 期子宫内膜异位症患者的子宫内膜容受性。

Transcriptomic analysis shows that surgical treatment is likely to influence the endometrial receptivity of patients with stage III/IV endometriosis.

机构信息

Reproductive Medicine Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Department of Gynecology and Obstetrics, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

出版信息

Front Endocrinol (Lausanne). 2022 Aug 29;13:932339. doi: 10.3389/fendo.2022.932339. eCollection 2022.

DOI:10.3389/fendo.2022.932339

PMID:36171908

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9511704/

Abstract

BACKGROUND

Endometriosis negatively affects fertility, and it is a common disease in assisted reproductive practice. Surgical removal of endometriotic lesions is widely carried out to relieve symptoms and promote fertility. But it is not intensively investigated what changes in the secretory eutopic endometrium of patients with endometriosis after surgery.

METHODS

Eighteen patients with stage III/IV endometriosis were included in the study, and they were divided into the untreated group and the treated group (6 vs. 12). Basic clinical data were compared, and transcriptomic data of the secretory eutopic endometrium were analyzed with DESeq2, Cytoscape, ClueGO, CluePedia, and Gene Set Enrichment Analysis (GSEA). CIBERSORT was used to calculate the relative abundance of 22 immune cells in the samples.

RESULTS

We determined 346 differentially expressed genes (DEGs) using DESeq2. These DEGs were used to enrich seven Gene Ontology terms including three associated with immune processes and one correlated to prostaglandin using ClueGO and CluePedia. GSEA enriched 28 Gene Ontology terms in the treated group mainly associated with immune and blood pressure regulation process. Compared to the untreated group, the relative abundance of resting CD4+ memory T cells [0.218 (0.069, 0.334) vs. 0.332 (0.181, 0.429), = 0.022] and the even less abundant memory B cells [0.001 (0.000, 0.083) vs. 0.033 (0.007, 0.057), = 0.049] are significantly decreased in the treated group.

CONCLUSION

Surgical treatment of stage III/IV endometriosis influences some genes and biological processes related to endometrial receptivity, but more evidence is needed.

摘要

背景

子宫内膜异位症会对生育能力产生负面影响，它是辅助生殖实践中的常见疾病。广泛开展手术切除子宫内膜异位病灶以缓解症状并提高生育能力。但是，对于手术后子宫内膜异位症患者的分泌型在位子宫内膜的变化，研究并不深入。

方法

本研究纳入了 18 名 III/IV 期子宫内膜异位症患者，将其分为未治疗组和治疗组（6 例 vs. 12 例）。比较了基本临床数据，并使用 DESeq2、Cytoscape、ClueGO、CluePedia 和基因集富集分析（GSEA）分析分泌型在位子宫内膜的转录组数据。使用 CIBERSORT 计算样本中 22 种免疫细胞的相对丰度。

结果

我们使用 DESeq2 确定了 346 个差异表达基因（DEGs）。这些 DEGs 用于富集 ClueGO 和 CluePedia 中的三个与免疫过程相关的基因本体论术语和一个与前列腺素相关的术语。GSEA 在治疗组中富集了 28 个基因本体论术语，主要与免疫和血压调节过程相关。与未治疗组相比，治疗组中静止 CD4+记忆 T 细胞的相对丰度[0.218（0.069，0.334）vs. 0.332（0.181，0.429）， = 0.022]和记忆 B 细胞的相对丰度甚至更低[0.001（0.000，0.083）vs. 0.033（0.007，0.057）， = 0.049]显著降低。