Shi Chenyang, Tang Yanan, Yang Hanyu, Yang Junfeng, Wu Yuyang, Sun Hang, Yin Shengyan, Wang Guangbin
State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, Jilin 130012, P. R. China.
Key Laboratory of Bionic Engineering (Ministry of Education), College of Biological and Agricultural Engineering, Jilin University, Changchun, Jilin 130022, P. R. China.
J Mater Chem B. 2022 Oct 19;10(40):8211-8217. doi: 10.1039/d2tb01749k.
Some pathogenic bacteria may cause serious food poisoning as well as catastrophic infections. Thus, it is critical to identify bacteria using simple, quick, and sensitive methods. Herein, we fabricate a graphene aerogel-based biosensing system to capture and detect () with high specificity and sensitivity. A graphene aerogel is prepared by a one-step hydrothermal synthesis method without any reducing reagent. With the help of antibodies and the graphene foam with a porous structure, can be captured using the detection substrate with high specificity and selectivity. The electrical resistance and electrochemical impedance spectroscopy (EIS) results of the graphene aerogel foam changed with high sensitivity during adhesion. Moreover, the resistance change of the graphene device can still be observed when the concentration was as low as 10 cfu mL, while there is no obvious resistance change in the use of . The subsequent EIS test also found that the charge transfer resistance () of the detection substrate gradually increased during the capture process. This nanoelectronic biosensor is simple, quick, safe, and very sensitive, and it may be used as a high-throughput platform for pathogenic bacterial detection, bacterial research, and antimicrobial drug screening.
一些致病细菌可能会导致严重的食物中毒以及灾难性感染。因此，使用简单、快速且灵敏的方法来鉴定细菌至关重要。在此，我们构建了一种基于石墨烯气凝胶的生物传感系统，以高特异性和灵敏度捕获并检测（）。石墨烯气凝胶通过一步水热合成法制备，无需任何还原剂。借助抗体和具有多孔结构的石墨烯泡沫，（）可被检测底物以高特异性和选择性捕获。在（）粘附过程中，石墨烯气凝胶泡沫的电阻和电化学阻抗谱（EIS）结果变化灵敏。此外，当（）浓度低至10 cfu/mL时，仍可观察到石墨烯器件的电阻变化，而在使用（）时没有明显的电阻变化。随后的EIS测试还发现，在（）捕获过程中，检测底物的电荷转移电阻（）逐渐增加。这种纳米电子生物传感器简单、快速、安全且非常灵敏，可作为用于致病细菌检测、细菌研究和抗菌药物筛选的高通量平台。
