Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
School of Pharmaceutical Chemical and Materials Engineering, Taizhou University, Jiaojiang, Taizhou 318000, China.
ACS Appl Mater Interfaces. 2022 Oct 12;14(40):46103-46111. doi: 10.1021/acsami.2c14376. Epub 2022 Sep 29.
Dual-mode bioanalysis integrating photoelectrochemical (PEC) and other modes is emerging and allows signal cross-checking for more reliable results. Metal-organic frameworks (MOFs) have been shown to be attractive materials in various biological applications. This work presents the utilization of MOF encapsulation and stimuli-responsive decapsulation for dual-mode PEC and fluorescence (FL) bioanalysis. Photoactive dye methylene violet (MV) was encapsulated in zeolitic imidazolate framework-90 (ZIF-90) to form an MV@ZIF-90 hybrid material, and MV could be released by adenosine triphosphate (ATP)-induced ZIF-90 disintegration. The released MV not only had FL emission but also had a sensitization effect on the ZnInS (ZnInS) photoanode. Based on the MV-dependent sensitization effect and FL emission characteristic, a dual-mode PEC-FL strategy was established for ATP detection with low detection limits, that is, 3.2 and 4.1 pM for PEC and FL detection, respectively. This study features and will inspire the construction and implementation of smart MOF materials for dual-mode bioanalysis.
双模生物分析整合光电化学（PEC）和其他模式正在出现，并允许信号交叉检查以获得更可靠的结果。金属有机骨架（MOF）已被证明在各种生物应用中是有吸引力的材料。本工作利用 MOF 封装和响应性解封装进行双模 PEC 和荧光（FL）生物分析。将活性染料亚甲紫（MV）封装在沸石咪唑骨架-90（ZIF-90）中形成 MV@ZIF-90 杂化材料，并且 MV 可以通过三磷酸腺苷（ATP）诱导的 ZIF-90 崩解释放。释放的 MV 不仅具有 FL 发射，而且对 ZnInS（ZnInS）光电阳极具有敏化作用。基于 MV 依赖性敏化效应和 FL 发射特性，建立了用于 ATP 检测的双模 PEC-FL 策略，PEC 和 FL 检测的检测限分别低至 3.2 和 4.1 pM。本研究的特点和启示将为双模生物分析构建和实施智能 MOF 材料。
