Hou Waner, Liu Zhimin, Hao Xuanxuan, Lei Xun, Cheng Jintao, Li Yaxin, Hu Leqian, Li Peng
College of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, People's Republic of China.
Institute for Complexity Science, Henan University of Technology, Zhengzhou, 450001, People's Republic of China.
Mikrochim Acta. 2025 May 23;192(6):368. doi: 10.1007/s00604-025-07205-x.
An electrochemiluminescence (ECL)-fluorescence (FL) dual-mode aptasensing system is proposed for estradiol detection. Firstly, ruthenium-based metal-organic framework nanosheets (RuMOFNSs) were synthesized by a simple one-pot method. RuMOFNSs exhibited both fluorescence (FL) and electrochemiluminescence (ECL) characteristics. After RuMOFNSs were immobilized on the electrode, complementary target DNA (cDNA), gold nanoclusters labeled aptamer (AuNCs-Apt) were successively assembled on the electrode to fabricate the aptasensor. The absorption spectrum of RuMOFNSs could be well overlapped with the fluorescence emission spectrum (or ECL spectrum) of AuNCs. When estradiol was absent, AuNCs-Apt hybridizes with cDNA to form double-stranded DNA. The close proximity between AuNCs and RuMOFNSs led to the efficient resonance energy transfer (RET) from AuNCs (donor) to RuMOFNSs (acceptor), thus the enhanced ECL (or FL) signals were achieved. In the presence of estradiol, the high affinity between Apt and estradiol led to the dissociation of double-stranded DNA; the increased distance between AuNCs and RuMOFNSs hindered the RET, and the decreased ECL (or FL) signals were obtained. The aptasensor demonstrates exceptional sensitivity for detecting estradiol, with FL and ECL detection limits of 97 pM and 20 fM, respectively. This innovative approach offers significant potential for endocrine-disrupting chemical determination.
提出了一种用于检测雌二醇的电化学发光（ECL）-荧光（FL）双模式适配体传感系统。首先，通过简单的一锅法合成了钌基金属有机框架纳米片（RuMOFNSs）。RuMOFNSs同时表现出荧光（FL）和电化学发光（ECL）特性。将RuMOFNSs固定在电极上后，互补靶DNA（cDNA）、金纳米簇标记的适配体（AuNCs-Apt）依次组装在电极上，构建适配体传感器。RuMOFNSs的吸收光谱可以与AuNCs的荧光发射光谱（或ECL光谱）很好地重叠。当不存在雌二醇时，AuNCs-Apt与cDNA杂交形成双链DNA。AuNCs与RuMOFNSs之间的紧密接近导致了从AuNCs（供体）到RuMOFNSs（受体）的高效共振能量转移（RET），从而实现了增强的ECL（或FL）信号。在存在雌二醇的情况下，Apt与雌二醇之间的高亲和力导致双链DNA解离；AuNCs与RuMOFNSs之间距离的增加阻碍了RET，从而获得降低的ECL（或FL）信号。该适配体传感器对雌二醇检测表现出卓越的灵敏度，FL和ECL检测限分别为97 pM和20 fM。这种创新方法在内分泌干扰化学物测定方面具有巨大潜力。
