Department of Diabetes, Endocrinology and Hematology, Dokkyo Medical University Saitama Medical Center, Koshigaya, Saitama, Japan.
Department of Diabetes and Endocrinology, National Disaster Medical Center, Tachikawa, Tokyo, Japan.
Methods Mol Biol. 2023;2577:269-277. doi: 10.1007/978-1-0716-2724-2_19.
A new technique called the dCas9-SunTag and scFv-TET1CD epigenome editing system has recently been developed to edit the DNA methylation status of specific genes. The transfection of an all-in-one vector containing this system into cells is feasible and induces the DNA demethylation of specific genes; however, due to the large size of the vector, difficulties are associated with its introduction into mice. We herein used a hydrodynamic tail vein injection (HTVi) to introduce the all-in-one vector into mice for in vivo epigenome editing. HTVi needs to be considered for inducing the targeted DNA demethylation of particular genes in the mouse liver.
一种名为 dCas9-SunTag 和 scFv-TET1CD 的表观基因组编辑系统的新技术最近被开发出来，用于编辑特定基因的 DNA 甲基化状态。将包含该系统的一体式载体转染到细胞中是可行的，并诱导特定基因的 DNA 去甲基化；然而，由于载体体积较大，将其引入小鼠体内存在困难。我们在此使用了一种流体力学尾静脉注射（HTVi）将一体式载体引入小鼠体内进行体内表观基因组编辑。HTVi 需要考虑用于诱导小鼠肝脏中特定基因的靶向 DNA 去甲基化。
