Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan.
Methods Mol Biol. 2023;2577:255-268. doi: 10.1007/978-1-0716-2724-2_18.
Epigenetic regulatory mechanisms play an important role in gene silencing and genome stability; therefore, epigenetic mutations cause a variety of diseases. Analysis of the epigenome by next-generation sequencers has revealed many epigenetic mutations in various diseases such as cancer, obesity, diabetes, autism, allergies, immune diseases, and imprinting diseases. Unfortunately, it has been difficult to identify the causative epigenetic mutations because there has been no method to generate animals with target-specific epigenetic mutations. However, it has become possible to generate such animals due to the recent development of epigenome editing technology. Here, we introduce the generation of epigenome-edited mice by target-specific DNA demethylation.
表观遗传调控机制在基因沉默和基因组稳定性中发挥着重要作用；因此，表观遗传突变会导致多种疾病。下一代测序仪对表观基因组的分析揭示了各种疾病（如癌症、肥胖症、糖尿病、自闭症、过敏、自身免疫性疾病和印记疾病）中的许多表观遗传突变。不幸的是，由于一直没有生成具有特定靶向性的表观遗传突变动物的方法，因此很难确定致病的表观遗传突变。然而，由于最近表观基因组编辑技术的发展，生成这样的动物成为可能。在这里，我们介绍通过靶向特异性 DNA 去甲基化来生成表观基因组编辑的小鼠。
