State Key Laboratory for Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, PR China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, PR China.
State Key Laboratory for Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, PR China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, PR China.
Fitoterapia. 2022 Nov;163:105314. doi: 10.1016/j.fitote.2022.105314. Epub 2022 Sep 27.
The ent-kaurane diterpenoid enriched fraction (EDEF) of maize root was isolated and purified, and 10 compounds, including 4 ent-kaurane diterpenoids, were isolated and identified. We evaluated their neuroprotective properties in vitro for the first time using an HO-induced oxidative damage model in SH-SY5Y cells. The results showed that pretreatment with maizediterpene D, a new ent-kaurane diterpenoid isolated from the EDEF, significantly attenuated HO-induced apoptosis by improving cell survival, reducing ROS production and increasing mitochondrial membrane potential. Mechanistically, the neuroprotective effect of maizediterpene D was confirmed to be related to the dual activation of IGF-1R and BDNF/TrkB crosstalk pathways. Our findings suggest that the EDEF and its active constituent maizediterpene D had good neuroprotective properties and could serve as potential candidates for the development of therapeutic drugs for oxidative stress-related diseases.
玉米根中富含贝壳杉烷二萜的部分（EDEF）被分离和纯化，分离并鉴定了 10 种化合物，其中包括 4 种贝壳杉烷二萜。我们首次使用 HO 诱导的 SH-SY5Y 细胞氧化损伤模型，在体外评估了它们的神经保护特性。结果表明，从 EDEF 中分离出的新贝壳杉烷二萜玉米萜烯 D 预处理可通过提高细胞存活率、降低 ROS 产生和增加线粒体膜电位显著减轻 HO 诱导的细胞凋亡。从机制上讲，玉米萜烯 D 的神经保护作用被证实与 IGF-1R 和 BDNF/TrkB 串扰途径的双重激活有关。我们的研究结果表明，EDEF 及其活性成分玉米萜烯 D 具有良好的神经保护特性，可能成为治疗与氧化应激相关疾病的药物的潜在候选药物。
