Chinese People's Liberation Army 210 Hospital , Dalian 116021 , People's Republic of China.
J Nat Prod. 2018 May 25;81(5):1225-1234. doi: 10.1021/acs.jnatprod.7b01017. Epub 2018 May 15.
Thirteen new ent-kaurane diterpenoids, stigmaydenes A-M (1-13), together with two known compounds (14, 15), were isolated from the crude extract of corn silk ( Zea mays). The structures of the compounds were confirmed by comprehensive spectroscopic analyses. The absolute configuration of compound 1 was defined by single-crystal X-ray diffraction. The absolute configurations of the compounds were also confirmed by comparison of experimental and calculated specific rotations. The compounds were evaluated for their neuroprotective effects against HO-induced SH-SY5Y cell injury, and compound 8 was active at 100 μM, as determined by flow cytometry (annexin V-FITC/PI staining) and Hoechst 33258 staining. The results suggested that compound 8 could protect neuronal cells from HO-induced injury by inhibiting apoptosis in SH-SY5Y cells.
从玉米须（Zea mays）粗提物中分离得到了 13 个新的-ent-贝壳杉烷二萜类化合物， stigmaydenes A-M（1-13），以及两个已知化合物（14、15）。通过综合光谱分析确定了化合物的结构。通过单晶 X 射线衍射确定了化合物 1 的绝对构型。通过比较实验和计算的比旋光度也确定了化合物的绝对构型。通过流式细胞术（Annexin V-FITC/PI 染色）和 Hoechst 33258 染色评估了这些化合物对 HO 诱导的 SH-SY5Y 细胞损伤的神经保护作用，结果表明化合物 8 在 100 μM 时具有活性，可通过抑制 SH-SY5Y 细胞中的细胞凋亡来保护神经元细胞免受 HO 诱导的损伤。
