College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.
Fish Shellfish Immunol. 2022 Dec;131:54-66. doi: 10.1016/j.fsi.2022.09.039. Epub 2022 Sep 26.
Rainbow trout (Oncorhynchus mykiss), an economically important cold-water fish cultured worldwide, suffers from infectious hematopoietic necrosis virus (IHNV) infection, resulting in huge financial losses. In order to understand the immune response of rainbow trout during virus infection, we explored trout intestine transcriptome profiles following IHNV challenge, and identified 3355 differentially expressed genes (DEGs) and 80 differentially expressed miRNAs (DEMs). Transcriptome analysis revealed numerous DEGs involved in immune responses, such as toll-like receptor 3 (TLR3), toll-like receptor 7/8 (TLR7/8), tripartite motif-containing 25 (TRIM25), DExH-Box helicase 58 (DHX58), interferon-induced with helicase C domain 1 (IFIH1), interferon regulatory factor 3 (IRF3/7), signal transducer and activator of transcription 1 (STAT1) and heat shock protein 90-alpha 1 (HSP90A1). Integrated analysis identified five key miRNAs (miR-19-y, miR-181-z, miR-203-y, miR-143-z and miR-206-y) targeting at least two important immune genes (TRIM25, DHX58, STAT1, TLR7/8 and HSP90A1). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that DEGs and target genes were significantly enriched in various immune-related terms including immune system process, binding, cell part and pathways of Toll-like receptor signalling, RIG-I-like receptor signalling, NOD-like receptor signalling, JAK-STAT signalling, PI3K-Akt signalling, NF-kappa B signalling, IL-17 signalling and AGE-RAGE signalling. In addition, protein-protein interaction networks (PPI) was used to display highly interactive DEG networks involving eight immune-related pathways. The expression trends of 12 DEGs and 10 DEMs were further verified by quantitative real-time PCR, which confirmed the reliability of the transcriptome sequencing results. This study expands our understanding of the immune response of rainbow trout infected with IHNV, and provides valuable resources for future studies on the immune molecular mechanism and disease resistance breeding.
虹鳟（Oncorhynchus mykiss）是一种经济价值很高的冷水鱼类，在全球范围内养殖，但其易感染传染性造血器官坏死病毒（IHNV），造成巨大的经济损失。为了了解虹鳟感染病毒时的免疫反应，我们对 IHNV 感染后虹鳟肠道的转录组图谱进行了研究，共鉴定到 3355 个差异表达基因（DEGs）和 80 个差异表达 miRNA（DEMs）。转录组分析揭示了大量参与免疫反应的 DEGs，如 Toll 样受体 3（TLR3）、Toll 样受体 7/8（TLR7/8）、三结构域包含蛋白 25（TRIM25）、解旋酶 58（DHX58）、干扰素诱导的含螺旋酶 C 域 1（IFIH1）、干扰素调节因子 3（IRF3/7）、信号转导和转录激活因子 1（STAT1）和热休克蛋白 90-α 1（HSP90A1）。整合分析确定了五个关键的 miRNA（miR-19-y、miR-181-z、miR-203-y、miR-143-z 和 miR-206-y），它们至少靶向两个重要的免疫基因（TRIM25、DHX58、STAT1、TLR7/8 和 HSP90A1）。GO 和 KEGG 分析表明，DEGs 和靶基因显著富集于各种免疫相关术语中，包括免疫系统过程、结合、细胞组分和 Toll 样受体信号转导、RIG-I 样受体信号转导、NOD 样受体信号转导、JAK-STAT 信号转导、PI3K-Akt 信号转导、NF-κB 信号转导、IL-17 信号转导和 AGE-RAGE 信号转导途径。此外，还利用蛋白质-蛋白质相互作用网络（PPI）展示了涉及 8 个免疫相关途径的高度相互作用的 DEG 网络。通过定量实时 PCR 进一步验证了 12 个 DEG 和 10 个 DEM 的表达趋势，这证实了转录组测序结果的可靠性。本研究扩展了我们对感染 IHNV 的虹鳟免疫反应的认识，为进一步研究免疫分子机制和抗病性育种提供了有价值的资源。
