Luo Jin, Tan Yangchun, Zhao Shuaiyang, Ren Qiaoyun, Guan Guiquan, Luo Jianxun, Yin Hong, Liu Guangyuan
State Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou 730046, China.
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Pathogens. 2024 Mar 28;13(4):288. doi: 10.3390/pathogens13040288.
Ticks are an important type of pathogen transmission vector, and pathogens not only cause serious harm to livestock but can also infect humans. Because of the roles that ticks play in disease transmission, reducing tick pathogen infectivity has become increasingly important and requires the identification and characterization of these pathogens and their interaction mechanisms. In this study, we determined the miRNA expression profile of infected with , predicted the target genes of miRNAs involved in this infection process, and investigated the role of miRNA target recognition during host-pathogen interactions. The results showed that longipain is a target gene of miR-5309, which was differentially expressed at different developmental stages and in various tissues in the control group. However, the miR-5309 level was reduced in the infection group. Analysis of the interaction between miRNA and the target gene showed that miR-5309 negatively regulated the expression of the longipain protein during the infection of with . To verify this inference, we compared longipain with the blocking agent orientalis. In this study, the expression of longipain was upregulated by the inhibition of miR-5309 in ticks, and the ability of the antibody produced by the tick-derived protein to attenuate infection was verified through animal immunity and antigen-antibody binding tests. The results showed that expression of the longipain + GST fusion protein caused the cattle to produce antibodies that could be successfully captured by ticks, and cellular immunity was subsequently activated in the ticks, resulting in a subtractive effect on infection. This research provides ideas for the control of ticks and tickborne diseases and a research basis for studying the mechanism underlying the interaction between ticks and pathogens.
蜱是一种重要的病原体传播媒介，病原体不仅会对家畜造成严重危害，还能感染人类。由于蜱在疾病传播中所起的作用，降低蜱传播病原体的能力变得越发重要，这需要对这些病原体进行鉴定和表征，并研究它们的相互作用机制。在本研究中，我们测定了感染[具体病原体名称缺失]的蜱的miRNA表达谱，预测了参与该感染过程的miRNA的靶基因，并研究了miRNA靶标识别在宿主-病原体相互作用中的作用。结果表明，longipain是miR-5309的靶基因，在对照组的不同发育阶段和各种组织中差异表达。然而，感染组中miR-5309水平降低。对miRNA与靶基因之间相互作用的分析表明，在蜱感染[具体病原体名称缺失]的过程中，miR-5309负向调节longipain蛋白的表达。为了验证这一推断，我们将longipain与阻断剂东方蝾螈进行了比较。在本研究中，通过抑制蜱中的miR-5309上调了longipain 的表达，并通过动物免疫和抗原-抗体结合试验验证了蜱源蛋白产生的抗体减轻[具体病原体名称缺失]感染的能力。结果表明，longipain + GST融合蛋白的表达使牛产生了可被蜱成功捕获的抗体，随后蜱中的细胞免疫被激活，从而对[具体病原体名称缺失]感染产生了消减作用。本研究为蜱及蜱传疾病的防治提供了思路，为研究蜱与病原体相互作用的机制提供了研究基础。
