State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.
J Proteomics. 2023 Jan 6;270:104736. doi: 10.1016/j.jprot.2022.104736. Epub 2022 Sep 27.
Seed longevity is important for the maintenance of seed nutritional quality, vigor, and germination potential during storage. Sacred lotus is known as one of the longest living seeds in the world and their ability to maintain longevity has been widely investigated. In this study, a suitable controlled deterioration treatment (CDT) method was first established to evaluate the vigor loss of lotus plumule (LP), and then the Tandem Mass Tags (TMT)-based proteomic analysis was performed on LP from the CDT-treated seed to quantitatively and qualitatively analyze the protein profile dynamic. In total, 4002 proteins were successfully quantified, of them, 558 differently accumulated proteins (DAPs) were identified. Protein processing and RNA-related proteins were found more easily to be affected by CDT, which may directly result in seed vigor loss. Meanwhile, CDT resulted in remarkable up-regulation of numerous proteins related to antioxidation, photosynthesis, RNA and DNA stability, starch and sucrose mobilization, and cell membrane and wall stability, which potentially played key roles in maintaining the lotus seed vigor under CDT. Histological and physiological analyses were also performed to verify some proteome results. This study provided both fundamental data and new insights to further uncover the secret of lotus seed longevity. SIGNIFICANCE: Seed aging affects the seed quality and can result in direct economic losses. The exceptional longevity of sacred lotus seed has attracted extensive attention. In this study, an optimized CDT method was used to mimic the natural aging process of sacred lotus seed, and based on TMT-based quantitative proteomic analysis on the LP profile of CDT-treated seeds, a series of differentially accumulation of specific proteins (DEPs) were revealed related to CDT resistance. Correspondingly, the physiological state and histological structure of the LP along with the CDT were detected to verify the proteome data. This study provided comprehensive information for the molecular basis of lotus seed aging analysis and facilitate to screen seed longevity related proteins for other plant species.
种子的长寿对于维持其在储存过程中的营养品质、活力和发芽潜力非常重要。 圣莲被认为是世界上寿命最长的种子之一，其保持长寿的能力已被广泛研究。 在这项研究中，首先建立了一种合适的受控劣化处理（CDT）方法来评估莲子活力的丧失，然后对 CDT 处理后的种子进行串联质量标签（TMT）-基于蛋白质组学分析，定量和定性分析蛋白质谱动态。 总共成功定量了 4002 种蛋白质，其中鉴定了 558 种差异积累蛋白（DAP）。 发现蛋白质加工和 RNA 相关蛋白更容易受到 CDT 的影响，这可能直接导致种子活力丧失。 同时，CDT 导致许多与抗氧化、光合作用、RNA 和 DNA 稳定性、淀粉和蔗糖动员以及细胞膜和细胞壁稳定性相关的蛋白质显著上调，这可能在维持 CDT 下的莲子活力方面发挥关键作用。 还进行了组织学和生理学分析来验证一些蛋白质组结果。 这项研究为进一步揭示莲子长寿的秘密提供了基础数据和新的见解。 意义：种子老化会影响种子质量，并导致直接的经济损失。 圣莲种子的超长寿命引起了广泛关注。 在这项研究中，使用优化的 CDT 方法模拟圣莲种子的自然老化过程，基于 TMT 定量蛋白质组分析 CDT 处理种子的 LP 谱，揭示了与 CDT 抗性相关的一系列特定蛋白质的差异积累（DEPs）。 相应地，检测 LP 沿 CDT 的生理状态和组织学结构以验证蛋白质组数据。 这项研究为莲子老化分析的分子基础提供了全面的信息，并有助于筛选其他植物物种的种子长寿相关蛋白。
