Nouemsi Guy Raphael Sado, Jouda Jean-Bosco, Leutcha Peron Bosco, Matieta Valaire Yemene, Tsague Tankeu Virginie Flaure, Ngnouzouba Kuete Jenifer Reine, Çelik İlhami, Kuete Victor, Sewald Norbert, Lannang Alain Meli
Department of Chemistry, Faculty of Science, University of Maroua, Maroua, Cameroon.
Department of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, Ngaoundere, Cameroon.
Nat Prod Res. 2023 Aug-Sep;37(16):2653-2661. doi: 10.1080/14786419.2022.2128347. Epub 2022 Sep 29.
Investigation of the leaves of Schum led to the isolation and identification of a new flavonol derivative, 6-C-methylquercetin-3,4'-dimethyl ether () together with eleven known compounds (-), with two of them ( and ) obtained as a mixture. Their structures were established by extensive spectroscopic analyses. Antibacterial activity of compound as well as the reference antibiotic, ciprofloxacin was tested on Gram-negative multidrug-resistant bacteria overexpressing active efflux pumps, and against methicillin-resistant strains of (MRSA). Samples were tested alone and in combination with an efflux pump inhibitor (EPI), phenylalanine-arginine--naphthylamide (PAßN). Results show that when compound was tested alone, its inhibitory effects were obtained on 7/10 tested bacteria with the highest MIC value of 128 µg/mL whilst in the presence of EPI, this activity significantly increase in all the 10 bacteria. An interesting antibacterial activity was obtained with compound against ATCC11296 (MIC of 4 µg/mL), KP55 and MRSA6 in the presence of the PaßN.
对舒姆叶的研究导致分离并鉴定出一种新的黄酮醇衍生物6 - C - 甲基槲皮素 - 3,4'- 二甲醚（）以及十一种已知化合物（ - ），其中两种（ 和 ）以混合物形式获得。通过广泛的光谱分析确定了它们的结构。在过表达活性外排泵的革兰氏阴性多重耐药细菌以及耐甲氧西林的金黄色葡萄球菌菌株（MRSA）上测试了化合物 以及参考抗生素环丙沙星的抗菌活性。单独测试样品，并与外排泵抑制剂（EPI）苯丙氨酸 - 精氨酸 - β - 萘酰胺（PAßN）联合测试。结果表明，单独测试化合物 时，对10种测试细菌中的7种有抑制作用，最高MIC值为128μg/mL，而在存在EPI的情况下，在所有10种细菌中该活性显著增加。在存在PaßN的情况下，化合物 对金黄色葡萄球菌ATCC11296（MIC为4μg/mL）、KP55和耐甲氧西林金黄色葡萄球菌MRSA6具有有趣的抗菌活性。
