Mambe Flora T, Na-Iya Jean, Fotso Ghislain W, Ashu Fred, Ngameni Bathélémy, Ngadjui Bonaventure T, Beng Veronique P, Kuete Victor
Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.
Evid Based Complement Alternat Med. 2019 Mar 7;2019:7507549. doi: 10.1155/2019/7507549. eCollection 2019.
The present study aimed to assess the antibacterial and antibiotic modifying activities of methanol extracts prepared from the leaf (APL) and bark (APB) of , fractions (APLa-d) and compounds isolated from APL against a panel of multidrug resistant (MDR) Gram-negative bacteria. Leaf extract was subjected to column chromatography for compounds isolation; antibacterial assays were performed on samples alone and with an efflux pump inhibitor (EPI), respectively, and several antibiotics on the tested bacteria. The phytochemical investigation of APL led to the isolation of stigmasterol (), -amyrin (), 3---glucopyranosylstigmasterol (), 3-O-methyl-D-chiro-inositol (), epicatechin (), quercetin-3--glucoside (), 3--[--xylopyranosyl-(1→4)---galactopyranosyl]-oleanolic acid (), and 3--[-galactopyranosyl-(1→4)---galactopyranosyl]-oleanolic acid (). APL and APB had minimal inhibitory concentration (MIC) values ≤ 1024 g/mL on 73.3% and 46.7% of the tested bacteria, respectively. APLb and APLd were effective against 88.9% of tested bacterial species with compound 8 showing the highest activity inhibiting 88.9% of tested bacteria. The EPI, phenylalanine-arginine--naphthylamide (PAßN), strongly improved the activity of APL, APLb, APLd, and compound on all tested bacteria. Synergistic effects were obtained when APL and compounds and were combined with erythromycin (ERY), gentamycin (GEN), ciprofloxacin (CIP), and norfloxacin (NOR). The present study demonstrates the antibacterial potential of and its constituents to combat bacterial infections alone or in combination with EPI.
本研究旨在评估从[植物名称]叶（APL）和树皮（APB）制备的甲醇提取物、APL的馏分（APLa - d）以及从APL中分离出的化合物对一组耐多药（MDR）革兰氏阴性菌的抗菌和抗生素修饰活性。叶提取物进行柱色谱分离化合物；分别对样品单独以及与外排泵抑制剂（EPI）一起进行抗菌试验，并对受试细菌使用了几种抗生素。对APL进行植物化学研究，分离出了豆甾醇（）、α - 香树脂醇（）、3 - O - β - 葡萄糖吡喃糖基豆甾醇（）、3 - O - 甲基 - D - 手性肌醇（）、表儿茶素（）、槲皮素 - 3 - O - 葡萄糖苷（）、3 - O - [β - 木糖吡喃糖基 - （1→4） - β - 半乳糖吡喃糖基] - 齐墩果酸（）和3 - O - [β - 半乳糖吡喃糖基 - （1→4） - β - 半乳糖吡喃糖基] - 齐墩果酸（）。APL和APB对73.3%和46.7%的受试细菌的最低抑菌浓度（MIC）值分别≤1024 μg/mL。APLb和APLd对88.9%的受试细菌有效，化合物8显示出最高活性，抑制了88.9%的受试细菌。EPI，苯丙氨酸 - 精氨酸 - β - 萘酰胺（PAβN），显著提高了APL、APLb、APLd和化合物对所有受试细菌的活性。当APL与化合物[具体化合物编号]和[具体化合物编号]分别与红霉素（ERY）、庆大霉素（GEN）、环丙沙星（CIP）和诺氟沙星（NOR）联合使用时，获得了协同效应。本研究证明了[植物名称]及其成分单独或与EPI联合对抗细菌感染的抗菌潜力。
