Sorbonne Université, Inserm U1135, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Immunology Department Hôpital Pitié-Salpêtrière, Paris, France.
Methods Mol Biol. 2023;2559:153-169. doi: 10.1007/978-1-0716-2647-4_11.
CD4 regulatory T (Treg) cells play an important role in maintaining immune homeostasis. Although these cells were initially studied as a homogenous cohort, we now know that they have unprecedented underlying heterogeneity. This heterogeneity is reflected in their phenotype and functions. As human Treg subpopulations are very small in numbers, it is necessary to develop novel ways of isolating and manipulating these cell populations. In this chapter, we discuss immunoassays established to this effect.
CD4 调节性 T（Treg）细胞在维持免疫稳态方面发挥着重要作用。尽管这些细胞最初被作为一个同质群体进行研究，但我们现在知道它们具有前所未有的潜在异质性。这种异质性反映在它们的表型和功能上。由于人类 Treg 亚群的数量非常少，因此有必要开发新的方法来分离和操纵这些细胞群体。在本章中，我们将讨论为此目的建立的免疫测定法。
