Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands; Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911, MA, Leeuwarden, The Netherlands.
Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911, MA, Leeuwarden, The Netherlands.
Bioresour Technol. 2022 Nov;364:128035. doi: 10.1016/j.biortech.2022.128035. Epub 2022 Sep 28.
The developments of mixed culture polyhydroxyalkanoate production has been directed to maximize the biomass PHA content with limited attention to polymer quality. Direct comparison of PHA accumulation literature is challenging, and even regularly contradicting in reported results, due to underlying differences that are not well expressed. A study was undertaken to systematically compare the commonly reported process conditions for PHA accumulation by full-scale municipal activated sludge. A biomass acclimation step combined with a pulse-wise feeding strategy resulted in maximum average PHA contents and product yields. pH control and active nitrification did not result in observable effects on the PHA productivity. Under these conditions a high molecular weight polymer (1536 ± 221 kDa) can be produced. Polymer extraction recoveries were influenced by the PHA molecular weight. A standard protocol for an activated sludge PHA accumulation test including downstream processing and standardized extraction has been developed and is available as supplementary material.
混合培养聚羟基烷酸酯生产的发展方向是最大限度地提高生物量PHA 的含量，而对聚合物质量的关注有限。由于潜在差异表达不充分，PHA 积累文献的直接比较具有挑战性，甚至经常在报告结果中相互矛盾。本研究旨在系统比较全规模城市活性污泥中 PHA 积累的常用报告工艺条件。生物量驯化步骤与脉冲进料策略相结合，可实现最大的平均 PHA 含量和产物产率。pH 值控制和活性硝化对 PHA 生产力没有明显影响。在此条件下，可以生产高分子量聚合物（1536±221 kDa）。聚合物提取回收率受 PHA 分子量的影响。已开发出一种包括下游处理和标准化提取的活性污泥 PHA 积累测试的标准方案，并作为补充材料提供。
