School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Centre for Rural Development and Technology, Indian Institute of Technology, New Delhi 110016, India.
Bioresour Technol. 2018 May;255:111-115. doi: 10.1016/j.biortech.2018.01.031. Epub 2018 Jan 8.
In the current study, the feasibility of utilizing municipal secondary wastewater sludge for Polyhydroxyalkanoate (PHA) extraction was improved by optimization of various parameters (temperature, duration and concentration of sludge solids). Optimized process parameters resulted in PHA recovery of 0.605 g, significantly higher than un-optimized conditions. The characterization of PHA was carried out by GC-MS, FT-IR and NMR (H and C) spectroscopy. The PHA profile was found to be dominated by mcl PHA (58%) along with other diverse PHA. The results of the present study show rich diversity of PHA extracted from a raw material which is readily available at minimal cost. In conclusion, exploring the potential of wastes for production of bioplastics not only reduces the cost of bioplastic production, but also provides a sustainable means for waste management.
在本研究中，通过优化各种参数（温度、污泥固体的持续时间和浓度），提高了利用城市二级废水污泥提取聚羟基烷酸酯（PHA）的可行性。优化后的工艺参数使 PHA 的回收量达到 0.605g，明显高于未优化条件下的回收量。通过 GC-MS、FT-IR 和 NMR（H 和 C）光谱对 PHA 进行了表征。PHA 图谱主要由 mcl PHA（58%）和其他各种 PHA 组成。本研究的结果表明，从一种原料中提取的 PHA 具有丰富的多样性，而这种原料很容易以最低的成本获得。总之，探索废物生产生物塑料的潜力不仅降低了生物塑料生产的成本，而且为废物管理提供了一种可持续的手段。
