多显微镜技术结合傅里叶变换红外光谱揭示了导致东方甜瓜（Cucumis melo var. Makuwa Makino）果实质地差异的组织学和生化原因。

Multi-microscopy techniques combined with FT-IR spectroscopy reveals the histological and biochemical causes leading to fruit texture difference in oriental melon (Cucumis melo var. Makuwa Makino).

机构信息

College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, PR China; Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, Liaoning 110866, PR China; Northern National & Local Joint Engineering Research Center of Horticultural Facilities Design and Application Technology (Liaoning), Shenyang, Liaoning 110866, PR China.

College of Horticulture, Henan Agricultural University, Zhengzhou, Henan 450002, PR China.

出版信息

Food Chem. 2023 Feb 15;402:134229. doi: 10.1016/j.foodchem.2022.134229. Epub 2022 Sep 14.

DOI:10.1016/j.foodchem.2022.134229

PMID:36182778

Abstract

Multi-microscopy techniques and Fourier transform infrared (FT-IR) spectroscopy were used in this study to investigate the intrinsic causes leading to fruit texture difference between two cultivars of oriental melon 'HDB' (crisp) and 'HPM' (mealy). On the histological aspect, orderly arranged regular-shaped cells with tissue natural fracture pattern showed cell rupture in 'HDB' versus loosely arranged irregular-shaped cells with tissue natural fracture pattern showed cell-to-cell separation in 'HPM' of sarcocarp are histological causes for crisp and mealy fruit texture, respectively. On the biochemical aspect, FT-IR spectra (4000-850 cm) of sarcocarp tissue cell wall materials (CWM) happened a dramatic change at the mature stage in 'HPM', but not in 'HDB'. Insightly, the lower de-methyl-esterified homogalacturonan (HG) abundance with higher water-soluble pectin (WSP) ratio and lower hemicellulose (HC) content contribute a poor intercellular adhesion in 'HPM' middle lamella (ML) at the mature stage compared to 'HDB'.

摘要

本研究采用多显微镜技术和傅里叶变换红外（FT-IR）光谱法，研究了导致两种东方甜瓜品种“HDB”（脆）和“HPM”（粉）果实质地差异的内在原因。在组织学方面，果肉中排列整齐的规则形状细胞和组织自然断裂模式表明细胞破裂，而排列松散的不规则形状细胞和组织自然断裂模式表明细胞分离，这分别是脆果和粉果质地的组织学原因。在生化方面，果肉细胞壁材料（CWM）的 FT-IR 光谱（4000-850 cm）在“HPM”成熟阶段发生了剧烈变化，但在“HDB”中没有发生。微观上，与“HDB”相比，“HPM”中层（ML）中较低的去甲酯化同质半乳糖醛酸聚糖（HG）丰度、较高的水溶性果胶（WSP）比例和较低的半纤维素（HC）含量导致细胞间黏附不良。