Sensor Research Unit (SRU), Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; National Nanotechnology Center of Advanced Structural and Functional Nanomaterials, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Program in Petrochemistry and Polymer Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Sensor Research Unit (SRU), Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; National Nanotechnology Center of Advanced Structural and Functional Nanomaterials, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
J Hazard Mater. 2023 Jan 15;442:130046. doi: 10.1016/j.jhazmat.2022.130046. Epub 2022 Sep 21.
In this work, we successfully developed an intriguing preparation strategy to reduce the size-dependent effect of nanoplastics (NPLs), which is the limitation of NPLs quantification by surface-enhanced Raman scattering (SERS). This simple and low-cost technique enabled us to quantify different sizes (i.e., 100, 300, 600, and 800 nm) of polystyrene nanospheres (PS NSs) in various aqueous media. The SERS substrate was simply prepared by sputtering gold particles to cover on a glass cover slide. By dissolving PS NSs in toluene and preconcentrating by coffee-ring effect, SERS measurement can quantify NPLs at a very low concentration with a limit of detection (LOD) of approximately 0.10-0.26 μg/mL. The experiment was also conducted in the presence of interferences, including salts, sugars, amino acids, and detergents. The method was validated for quantitative analysis using a mixture of 100-, 300-, 600-, and 800-nm PS NSs in a ratio of 1:1:1:1 in real-world media (i.e., tap water, mineral water, and river water), which successfully approaches the evaluation of PS NSs in the range of 10-40 µg/mL with an LOD of approximately 0.32-0.52 µg/mL.
在这项工作中，我们成功开发了一种有趣的制备策略，以减少纳米塑料（NPLs）的尺寸依赖性效应，这是表面增强拉曼散射（SERS）定量 NPLs 的限制。这种简单且低成本的技术使我们能够在各种水介质中定量不同尺寸（即 100、300、600 和 800nm）的聚苯乙烯纳米球（PS NSs）。SERS 基底通过溅射金颗粒简单制备，覆盖在玻璃盖玻片上。通过将 PS NSs 溶解在甲苯中并通过咖啡环效应预浓缩，可以通过 SERS 测量以低浓度定量 NPLs，检测限（LOD）约为 0.10-0.26μg/mL。该实验还在存在干扰的情况下进行，包括盐、糖、氨基酸和洗涤剂。该方法使用真实介质（即自来水、矿泉水和河水）中 100nm、300nm、600nm 和 800nm PS NSs 的比例为 1:1:1:1 的混合物进行了定量分析的验证，成功地在 10-40μg/mL 的范围内评估了 PS NSs，LOD 约为 0.32-0.52μg/mL。
