Zhao Yabo, Zhang Yanmei, Khas Erdene, Ao Changjin, Bai Chen
Key Laboratory of Animal Feed and Nutrition of Inner Mongolia Autonomous Region, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
Front Microbiol. 2022 Sep 15;13:978057. doi: 10.3389/fmicb.2022.978057. eCollection 2022.
The objective of this study was to evaluate the effect of Regel ethanol extract (AME) on the concentration of three branched-chain fatty acids (BCFAs) related to flavor, fermentation parameters and the bacteria and their correlations in the rumen of lambs. A total of thirty 3-month-old male, Small-tailed Han sheep (33.60 ± 1.23 kg) were randomly distributed into 2 groups as follows: control group (CON) was fed a basal diet and AME group was fed a basal diet supplemented with 2.8 g⋅lamb⋅d Regel ethanol extract. AME supplementation decreased ( = 0.022) 4-methyloctanoic acid (MOA) content and tended to lower ( = 0.055) 4-methylnonanoic acid (MNA) content in the rumen. Compared to CON group, the ruminal concentrations of valerate and isovalerate were higher ( = 0.046 and = 0.024, respectively), and propionate was lower ( = 0.020) in the AME group. At the phylum level, the AME group had a lower abundance of ( = 0.014) and a higher abundance of ( = 0.020) than the CON group. At the genus level, the relative abundances of ( = 0.001), ( = 0.003), ( = 0.004), and ( = 0.001) were significantly lower in the AME group than in the CON group, while the relative abundances of ( < 0.001), ( = 0.013), and ( = 0.001) were significantly higher. The relative abundances of ( = 0.029, = 0.685; = 0.009, = 0.770), ( = 0.019, = 0.721; = 0.029, = 0.685), and ( = 0.002, = 0.842; = 0.001, = 0.879) was positively correlated with MOA and MNA levels, and the relative abundance of ( = 0.033, = -0.673) was negatively correlated with MOA. The relative abundance of ( = 0.014, = -0.744) and ( = 0.023, = -0.706) correlated negatively with the EOA content. In conclusion, these findings suggest that the AME affected the concentration of BCFAs, fermentation parameters and the rumen bacteria in the rumen of lambs.
本研究的目的是评估 Regel 乙醇提取物（AME）对与风味相关的三种支链脂肪酸（BCFAs）浓度、发酵参数以及羔羊瘤胃中的细菌及其相关性的影响。总共 30 只 3 月龄的雄性小尾寒羊（体重 33.60 ± 1.23 千克）被随机分为 2 组，如下：对照组（CON）饲喂基础日粮，AME 组饲喂补充了 2.8 克·羔羊·天 Regel 乙醇提取物的基础日粮。补充 AME 降低了（P = 0.022）瘤胃中 4 - 甲基辛酸（MOA）的含量，并倾向于降低（P = 0.055）4 - 甲基壬酸（MNA）的含量。与 CON 组相比，AME 组瘤胃中戊酸和异戊酸的浓度较高（分别为 P = 0.046 和 P = 0.024），而丙酸浓度较低（P = 0.020）。在门水平上，AME 组的[某菌门]丰度低于 CON 组（P = 0.014），而[另一菌门]丰度高于 CON 组（P = 0.020）。在属水平上，AME 组中[某些菌属]的相对丰度（分别为 P = 0.001、P = 0.003、P = 0.004 和 P = 0.001）显著低于 CON 组，而[另一些菌属]的相对丰度（P < 0.001、P = 0.013 和 P = 0.001）显著更高。[某些菌属]的相对丰度（分别为 P = 0.029，r = 0.685；P = 0.009，r = 0.770）、[另一些菌属]的相对丰度（分别为 P = 0.019，r = 0.721；P = 0.029，r = 0.685）以及[还有些菌属]的相对丰度（分别为 P = 0.002，r = 0.842；P = 0.001，r = 0.879）与 MOA 和 MNA 水平呈正相关，而[某菌属]的相对丰度（P = 0.033，r = -0.673）与 MOA 呈负相关。[某些菌属]的相对丰度（分别为 P = 0.014，r = -0.744）和[另一些菌属]的相对丰度（P = 0.023，r = -0.706）与 EOA 含量呈负相关。总之，这些发现表明 AME 影响了羔羊瘤胃中 BCFAs 的浓度、发酵参数和瘤胃细菌。
