Mahobiya Sunil Kumar, Balayan Sapna, Chauhan Nidhi, Khanuja Manika, Kuchhal Naresh K, Islam S S, Jain Utkarsh
Amity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Sector 125, Noida 201313, Uttar Pradesh, India.
Centre for Nanoscience and Nanotechnology, Jamia Millia Islamia, New Delhi 110025, India.
ACS Omega. 2022 Sep 14;7(38):34676-34684. doi: 10.1021/acsomega.2c04926. eCollection 2022 Sep 27.
Diabetes is a global menace, and its severity results in various disorders including cardiovascular, retinopathy, neuropathy, and nephropathy. Recently, diabetic conditions are diagnosed through the level of glycated hemoglobin. The level of glycated hemoglobin is determined with enzymatic methodology. Although the system is sensitive, it has various restrictions such as long processing times, expensive equipment required for testing, and complex steps involved in sample preparation. These limitations are a hindrance to faster results. The limitations of the developed methods can be eliminated through biosensors. In this work, an electrochemical platform was fabricated that facilitates the identification of glycated hemoglobin protein in diabetic patients. The working electrode on the integrated circuit was modified with molecularly imprinted polymer decorated with tungsten disulfide nanoparticles to enhance its analytical properties. The analytical properties of the biosensor were studied using electrochemical techniques. The obtained detection limit of the nanoelectronic sensor was 0.01 pM. The calculated sensitivity of the biosensor was observed to be 0.27 μA/pM. Also, the sensor promises to operate in a dynamic working concentration range and provide instant results.
糖尿病是一种全球性威胁，其严重性会导致包括心血管疾病、视网膜病变、神经病变和肾病在内的各种疾病。最近，糖尿病状况是通过糖化血红蛋白水平来诊断的。糖化血红蛋白水平是用酶法测定的。尽管该系统很灵敏，但它有各种限制，如处理时间长、测试所需设备昂贵以及样品制备步骤复杂。这些限制阻碍了更快地获得结果。已开发方法的局限性可以通过生物传感器消除。在这项工作中，构建了一个电化学平台，便于识别糖尿病患者体内的糖化血红蛋白蛋白。集成电路上的工作电极用装饰有二硫化钨纳米颗粒的分子印迹聚合物进行了修饰，以增强其分析性能。使用电化学技术研究了生物传感器的分析性能。所获得的纳米电子传感器的检测限为0.01皮摩尔。观察到生物传感器的计算灵敏度为0.27微安/皮摩尔。此外，该传感器有望在动态工作浓度范围内运行并提供即时结果。
