Zhang Xiaojun, Wu Wensi, Zheng Zhenzhen, Li Liang, Chen Junjun, Zhong Junying, Zhao Le, Chen Jiawei, Wang Zhi, Meng Fanqing
Department of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Department of Thoracic Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, China.
Front Neurosci. 2022 Sep 15;16:990333. doi: 10.3389/fnins.2022.990333. eCollection 2022.
Prolonged exposure to general anesthesia (GA) results in long-lasting cognitive impairment, especially during critical stages of brain development. An exaggerated neuroinflammation induced by anesthesia is generally considered to be a key cause of cognitive impairment.
Postnatal day 7 (PND 7) mice were exposed to GA by isoflurane inhalation for 6 h or mock anesthesia. Disodium cromoglycate (DSCG) was intraperitoneally injected daily for 2 weeks, beginning from 30 min before anesthesia. The post-anesthesia evaluation included behavioral tests, toluidine blue staining, immunofluorescence and western blot.
Our results demonstrated the long-term cognition were impaired after 6 h GA exposure in neonatal mice. DSCG treatment ameliorated early mast cells (MCs) degranulation and mast cell tryptase (MCT) expression, which helps to attenuate subsequent neuroinflammation, activation of microglia and astrocytes, and damage to oligodendrocytes and synapses to improve cognitive impairment.
Disodium cromoglycate could effectively improve long-term cognitive impairment after GA exposure in neonatal mice.
长时间暴露于全身麻醉（GA）会导致持久的认知障碍，尤其是在大脑发育的关键阶段。麻醉引起的过度神经炎症通常被认为是认知障碍的关键原因。
出生后第7天（PND 7）的小鼠通过吸入异氟烷暴露于GA 6小时或进行假麻醉。从麻醉前30分钟开始，每天腹腔注射色甘酸钠（DSCG），持续2周。麻醉后评估包括行为测试、甲苯胺蓝染色、免疫荧光和蛋白质免疫印迹法。
我们的结果表明，新生小鼠暴露于GA 6小时后长期认知能力受损。DSCG治疗改善了早期肥大细胞（MCs）脱颗粒和肥大细胞类胰蛋白酶（MCT）表达，这有助于减轻随后的神经炎症、小胶质细胞和星形胶质细胞的激活以及少突胶质细胞和突触的损伤，从而改善认知障碍。
色甘酸钠可有效改善新生小鼠GA暴露后的长期认知障碍。
