色甘酸钠对人空肠黏膜肥大细胞体外脱颗粒的影响。

The effect of disodium cromoglycate on in vitro mast cell degranulation in human jejunal mucosa.

作者信息

Selbekk B H

出版信息

Allergy. 1979 Oct;34(5):283-8. doi: 10.1111/j.1398-9995.1979.tb04369.x.

DOI:10.1111/j.1398-9995.1979.tb04369.x

Abstract

The in vitro effect of disodium cromoglycate (DSCG) on IgE antigen-induced mast cell degranulation is described. Serum from an egg white allergic patient was used to sensitize jejunal mucosa from nine individuals. Egg white was used to challenge the IgE sensitized mast cells. DSCG in concentrations 3 x 10(-7) M to 3 x 10(-4) M was added to the mucosal specimens before antigen challenge. Mast cell degranulation in the sensitized specimens challenged with egg white was 38%. Mast cell degranulation in sensitized specimens treated with DSCG before and during antigen challenge was reduced to 2% at a concentration of 3 x 10(-5) M of DSCG (P=0.006) and to 28% at a concentration of 3 x 10(-6) M (P=0.027). No significant reduction of mast cell degranulation was seen at concentrations of 3 x 10(-7) M and 3 x 10(-4) M. The results support a role for DSCG in the treatment of gastrointestinal allergy.

摘要

本文描述了色甘酸钠（DSCG）对IgE抗原诱导的肥大细胞脱颗粒的体外作用。使用一名蛋清过敏患者的血清对9名个体的空肠黏膜进行致敏。用蛋清刺激IgE致敏的肥大细胞。在抗原刺激前，将浓度为3×10⁻⁷M至3×10⁻⁴M的DSCG添加到黏膜标本中。用蛋清刺激的致敏标本中肥大细胞脱颗粒率为38%。在抗原刺激前和刺激期间用DSCG处理的致敏标本中，当DSCG浓度为3×10⁻⁵M时，肥大细胞脱颗粒率降至2%（P = 0.006），当浓度为3×10⁻⁶M时降至28%（P = 0.027）。在3×10⁻⁷M和3×10⁻⁴M浓度下，未观察到肥大细胞脱颗粒有显著降低。结果支持DSCG在治疗胃肠道过敏中发挥作用。