Liu Qiao, Shen Jia-Man, Hong Hui-Jie, Yang Qi, Liu Wen, Guan Zhong, Wang Yi-Tao, Chen Xiao-Jia
State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Taipa, Macao SAR, China.
Front Pharmacol. 2022 Sep 16;13:1017830. doi: 10.3389/fphar.2022.1017830. eCollection 2022.
(OJ) is a traditional Chinese herbal medicine that has been used for thousands of years. Recently, the anticancer effects of OJ have been reported in multiple types of cancer, particularly in lung cancer. However, the underlying mechanisms remain unclear. In present study, the effects of OJ against NCI-H1299 human lung cancer cells were investigated, and the underlying mechanisms were explored using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS)-based cell metabolomics. As a result, OJ inhibited the proliferation, induced the apoptosis and suppressed the migration of NCI-H1299 cells. A total of 22 differential metabolites responsible for the effects of OJ were screened and annotated based on the LC-MS-based cell metabolomics approach. The altered metabolites were involved in three metabolic pathways, including glycerophospholipid metabolism, ether lipid metabolism and glutathione metabolism. These results showed that cell metabolomics-based strategies are promising tools to discover the action mechanisms of OJ against lung cancer cells.
莪术是一种已使用数千年的传统中药。最近，莪术在多种癌症，尤其是肺癌中的抗癌作用已有报道。然而，其潜在机制仍不清楚。在本研究中，研究了莪术对NCI-H1299人肺癌细胞的作用，并使用基于超高效液相色谱-四极杆飞行时间质谱（UHPLC/Q-TOF-MS）的细胞代谢组学方法探索其潜在机制。结果显示，莪术抑制了NCI-H1299细胞的增殖，诱导了细胞凋亡，并抑制了细胞迁移。基于基于液相色谱-质谱的细胞代谢组学方法，共筛选并注释了22种对莪术作用起作用的差异代谢物。这些变化的代谢物涉及三种代谢途径，包括甘油磷脂代谢、醚脂代谢和谷胱甘肽代谢。这些结果表明，基于细胞代谢组学的策略是发现莪术抗肺癌细胞作用机制的有前途的工具。
