Department of Clinical Laboratory, Jinling Hospital, State Key Laboratory of Analytical Chemistry for Life Science, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing University, Nanjing, China.
Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Chinese Academy of Medical Sciences Research Unit of Extracellular RNA, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, School of Life Sciences, Nanjing University, Nanjing, China.
Front Cell Infect Microbiol. 2022 Sep 15;12:992775. doi: 10.3389/fcimb.2022.992775. eCollection 2022.
Piwi-interacting RNAs (piRNAs) have emerged as potential novel indicators for various diseases; however, their diagnostic value for brucellosis remains unclear. This study aimed to evaluate the diagnostic potential of altered serum piRNAs in patients with brucellosis.
Illumina sequencing synthesis (SBS) technology was used to screen the serum piRNA profile in brucellosis patients, and markedly dysregulated piRNAs were confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) assay in two sets from a cohort of 73 brucellosis patients and 65 controls.
Illumina SBS technology results showed that seven piRNAs were markedly elevated in brucellosis patients compared to normal controls. The seven upregulated piRNAs were further validated individually by qRT-PCR, of which three piRNAs (piR-000753, piR-001312, and piR-016742) were confirmed to be significantly and steadily increased in the patients (> 2-fold, P < 0.01). The area under the receiver operating characteristic (ROC) curve (AUCs) for the three piRNAs ranged from 0.698 to 0.783. The AUC for the three piRNAs combination was 0.772, with a specificity of 86% and a positive predictive value of 90%, respectively.
The three-piRNA panel identified in this study has potential as a novel blood-based auxiliary tool for brucellosis detection.
Piwi 相互作用 RNA（piRNA）已成为各种疾病潜在的新型标志物；然而，其在布鲁氏菌病中的诊断价值尚不清楚。本研究旨在评估血清 piRNA 变化在布鲁氏菌病患者中的诊断潜力。
采用 Illumina 测序合成（SBS）技术筛选布鲁氏菌病患者血清 piRNA 谱，并用两组 73 例布鲁氏菌病患者和 65 例对照的定量实时聚合酶链反应（qRT-PCR）验证明显失调的 piRNA。
Illumina SBS 技术结果显示，与正常对照相比，7 种 piRNA 在布鲁氏菌病患者中明显升高。通过 qRT-PCR 进一步单独验证了这 7 个上调的 piRNA，其中 3 个 piRNA（piR-000753、piR-001312 和 piR-016742）在患者中显著且稳定增加（>2 倍，P < 0.01）。这 3 个 piRNA 的受试者工作特征（ROC）曲线下面积（AUCs）范围为 0.698 至 0.783。这 3 个 piRNA 组合的 AUC 为 0.772，特异性为 86%，阳性预测值为 90%。
本研究中鉴定的三 piRNA 谱可能成为一种新的基于血液的布鲁氏菌病检测辅助工具。
