Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, Guangdong Province, China.
Key Laboratory of Synthetic Rubber & Laboratory of Advanced Power Sources, Changchun Institute of Applied Chemistry (CIAC), Chinese Academy of Sciences, Changchun 130022, Jilin Province, China.
ACS Appl Mater Interfaces. 2022 Oct 26;14(42):47345-47358. doi: 10.1021/acsami.2c09348. Epub 2022 Oct 3.
Artificial recapitulation of hierarchically porous films gained great interest due to their versatile functionalities and applications. However, the development of novel eco-friendly and nontoxic biopolymer-based porous films is still limited by the time-consuming fabrication processes and toxic organic reagents involved. Here, we reported a novel approach to rapidly (within 5 s) fabricate biopolymer-based hierarchically porous films via inducing the laterally occurring interfacial self-assembly of prolamins at the air-liquid interface during an antisolvent dripping procedure. The as-prepared films exhibited a hierarchically porous microstructure (with sizes of about 500 nm to 5 μm) with location-graded and Janus features. The formation mechanism involved the solvent gradient controlled self-assembly of prolamin into an anisotropic defect structure in longitudinal and lateral directions. Accordingly, the macroscopic morphologies together with the porosity and pore size could be precisely tuned by solvents and operating parameters in a convenient way. Furthermore, alcohol-soluble but water-insoluble bioactive compounds could be incorporated simultaneously via a one-step loading procedure, which endowed films with large loading efficiency and sustained release features suitable for controlled release applications. The effect of the curcumin-loaded porous film on skin wound healing, as one of the potential applications of this novel material, was then investigated in a full-thickness wound model, wherein satisfying wound healing effects were achieved through multitarget and multipathway mechanisms. This pioneering work offers a novel strategy for the rapid architecture of biopolymer-based hierarchically porous film with versatile application potentials.
由于具有多功能性和广泛的应用，分层多孔膜的人工再现引起了极大的关注。然而，新型环保无毒的生物聚合物基多孔膜的发展仍然受到耗时的制造工艺和涉及的有毒有机试剂的限制。在这里，我们报道了一种通过在抗溶剂滴注过程中在气液界面诱导脯氨酸的侧向界面自组装来快速（5 秒内）制造基于生物聚合物的分层多孔膜的新方法。所制备的薄膜具有分层多孔微结构（尺寸约为 500nm 至 5μm），具有位置分级和詹纳斯特征。形成机制涉及溶剂梯度控制脯氨酸在纵向和横向方向上自组装成各向异性缺陷结构。因此，宏观形态以及孔隙率和孔径可以通过溶剂和操作参数以方便的方式进行精确调节。此外，通过一步装载程序可以同时掺入醇溶性但水溶性的生物活性化合物，这使薄膜具有大的负载效率和适合控制释放应用的持续释放特性。然后在全厚度伤口模型中研究了负载姜黄素的多孔膜对皮肤伤口愈合的影响，作为该新型材料的潜在应用之一，通过多靶点和多途径机制实现了令人满意的伤口愈合效果。这项开创性的工作为具有多种应用潜力的基于生物聚合物的分层多孔膜的快速构建提供了一种新策略。
